{"EMPIAR-12094":{"imagesets":[{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs","directory":"data/corrected_micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":93510,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":"data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs were obtained from movie-stacks of 20 frames à 2-2.5 e/A^2 by global motion-correction and dose-weighting using Relion 4.0, CTF parameters were estimated with GCTF 1.06 and particles were selected by GAUTOMATCH 0.56.\n- Micrographs star-file (Micrographs with FinalResolution better 4.5A): data/corrected_micrographs.star \n- Particle coordinates: data/corrected_micrographs/*_automatch.star","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Shiny particles, obtained by Bayesian polishing in Relion 4.0","directory":"data/particles","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":1308019,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":"data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc","picked_particles_file_pattern":"data/particles/shiny_particles360/job*/*/FoilHole_*_Data_*_Fractions_shiny.mrcs","picked_particles_directory":"data/particles/shiny_particles360/job*/*","details":"Star-files for individual particle populations are located in data/particles (see below), masks used for sorting and final refinements in data/particles/masks360.\nStar-file for all shiny particles:\n- data/shiny_particles360_all.star\nStar-files for shiny particles of major states\n- data/particles/shiny_particles360_48S-1.star\n- data/particles/shiny_particles360_48S-2.star\n- data/particles/shiny_particles360_48S-3.star\n- data/particles/shiny_particles360_48S-4.star\n- data/particles/shiny_particles360_48S-5.star\nStar-files for shiny particles of eIF2, tRNA and eIFB5 substates:\n- data/particles/shiny_particles360_48S-2-1.star\n- data/particles/shiny_particles360_48S-2-2.star\n- data/particles/shiny_particles360_48S-2-3.star\n- data/particles/shiny_particles360_48S-3-1.star\n- data/particles/shiny_particles360_48S-3-2.star\n- data/particles/shiny_particles360_48S-3-3.star\n- data/particles/shiny_particles360_48S-4-1.star\n- data/particles/shiny_particles360_48S-4-2.star\n- data/particles/shiny_particles360_48S-5-1.star\n- data/particles/shiny_particles360_48S-5-2.star\n- data/particles/shiny_particles360_48S-5-3.star\nStar-files for shiny particles of eIF3bi substates:\n- data/particles/shiny_particles360_48S-1_eIF3bi_majorstate.star\n- data/particles/shiny_particles360_closed48S_eIF3bi_majorstate.star\n- data/particles/shiny_particles360_closed48S_eIF3bi_minorstate1.star\n- data/particles/shiny_particles360_closed48S_eIF3bi_minorstate2.star","image_width":"360","image_height":"360"},{"segmentations":[],"name":"Signal-subtracted particles - eIF3core in open 48S","directory":"data/subtracted_particles/subtracted_particles240_eIF3core_open48S","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":92749,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":"data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc","picked_particles_file_pattern":"data/subtracted_particles/subtracted_particles240_eIF3core_open48S/subtracted_rank*_opticsgroup*.mrcs","picked_particles_directory":"data/subtracted_particles/subtracted_particles240_eIF3core_open48S","details":"Star-files with subtracted particles used for local refinement of eIF3core in open 48S (48S-1):\n- data/subtracted_particles/subtracted_particles240_eIF3core_open48S.star\nMasks used for refinements are located in data/subtracted_particles/masks240.","image_width":"240","image_height":"240"},{"segmentations":[],"name":"Signal-subtracted particles - eIF3core in closed 48S","directory":"data/subtracted_particles/subtracted_particles240_eIF3core_closed48S","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":356632,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.16,"pixel_height":1.16,"micrographs_file_pattern":"data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc","picked_particles_file_pattern":"data/subtracted_particles/subtracted_particles240_eIF3core_closed48S/subtracted_rank*_opticsgroup*.mrcs","picked_particles_directory":"data/subtracted_particles/subtracted_particles240_eIF3core_closed48S","details":"Star-files with subtracted particles used for local refinement of eIF3core in closed 48S (48S-2 to 5):\n- data/subtracted_particles/subtracted_particles240_eIF3core_closed48S.star\nMasks used for refinements are located in data/subtracted_particles/masks240.","image_width":"240","image_height":"240"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Structural basis for translational control by the human 48S initiation complex","principal_investigator":[{"author_orcid":"0000-0002-4609-4052","middle_name":null,"organization":"Max Planck Institute for Multidisciplinary Sciences","street":"Am Fassberg 11","town_or_city":"Goettingen","state_or_province":"Niedersachsen","post_or_zip":"37077","telephone":"495512012960","fax":"5512012960","first_name":"Niels","last_name":"Fischer","email":"niels.fischer [at] mpinat.mpg.de","country":"Germany","entry":"EMPIAR-12094"}],"status":"REL","deposition_date":"2024-02-16","release_date":"2025-05-04","obsolete_date":null,"update_date":"2026-03-07","corresponding_author":{"author":{"author_orcid":"0000-0002-4609-4052","middle_name":null,"organization":"Max Planck Institute for Multidisciplinary Sciences","street":"Am Fassberg 11","town_or_city":"Goettingen","state_or_province":"Niedersachsen","post_or_zip":"37077","first_name":"Niels","last_name":"Fischer","country":"Germany"}},"authors":[{"author":{"name":"Petrychenko V","author_orcid":"0000-0001-9867-9493"}},{"author":{"name":"Yi SH","author_orcid":"0000-0002-5671-637X"}},{"author":{"name":"Liedtke D","author_orcid":"0009-0009-0127-631X"}},{"author":{"name":"Peng BZ","author_orcid":"0000-0002-9832-8447"}},{"author":{"name":"Rodnina MV","author_orcid":"0000-0003-0105-3879"}},{"author":{"name":"Fischer N","author_orcid":"0000-0002-4609-4052"}}],"cross_references":["EMD-17697","EMD-17700","EMD-19128","EMD-17699","EMD-17696","EMD-17701","EMD-17698"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Petrychenko V","author_orcid":"0000-0001-9867-9493"},{"name":"Yi SH","author_orcid":"0000-0002-5671-637X"},{"name":"Liedtke D","author_orcid":"0009-0009-0127-631X"},{"name":"Peng BZ","author_orcid":"0000-0002-9832-8447"},{"name":"Rodnina MV","author_orcid":"0000-0003-0105-3879"},{"name":"Fischer N","author_orcid":"0000-0002-4609-4052"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis for translational control by the human 48S initiation complex","volume":"32","country":"","first_page":"62","last_page":"72","year":"2024","language":"English","doi":"10.1038/s41594-024-01378-4","pubmedid":"39289545","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":"1","preprint":false}],"dataset_size":"6.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8pj2","8pj5","8rg0","8pj4","8pj1","8pj6","8pj3"],"entry_doi":"10.6019/EMPIAR-12094"}}