{"EMPIAR-12081":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of PARP1-DNA-EB47 on graphene by vitrobot","directory":"data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":24130,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.069,"pixel_height":1.069,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The movies were imaged (Titan Krios) at a nominal pixel resolution of 1.069 A/pix using a Gatan K3 direct electron detector in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 26.38 e-/Å^2/s with a total electron dose of 52.77 e-/Å^2.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by vitrobot","directory":"data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":9643,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.751,"pixel_height":0.751,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.18 e-/Å^2/s with a total electron dose of 43.08 e-/Å^2.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless (crosslinked) on graphene by vitrobot","directory":"data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":8600,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.0691,"pixel_height":1.0691,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The movies were imaged (Titan Krios) at a nominal pixel resolution of 1.069 A/pix using a Gatan K3 direct electron detector in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 26.38 e-/Å^2/s with a total electron dose of 52.77 e-/Å^2.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless (crosslinked) on graphene by vitrobot; tilted collection","directory":"data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":15299,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.069,"pixel_height":1.069,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The movies were imaged (Titan Krios) at a nominal pixel resolution of 1.069 A/pix using a Gatan K3 direct electron detector (Thermo Fisher Scientific) in counting mode at a tilt of 30 degrees. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 26.2 e-/Å^2/s with a total electron dose of 83.82 e-/Å^2.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by Chameleon; grid1","directory":"data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":9643,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.7511,"pixel_height":0.7511,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.47 e-/Å^2/s with a total electron dose of 44.81 e-/Å^2.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by Chameleon; grid2","directory":"data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":7445,"frames_per_image":70,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.7511,"pixel_height":0.7511,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.90 e-/Å^2/s with a total electron dose of 55.27 e-/Å^2.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless in open-hole by Chameleon","directory":"data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4246,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.548,"pixel_height":0.548,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The movies were imaged (Talos Arctica) at a nominal pixel resolution of 0.548 A/pix using a K2 Summit direct electron detector (Gatan) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) with a total electron dose of 50.44 e-/Å^2.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Movies of PARP1-DNA Complexes","principal_investigator":[{"author_orcid":"0000-0002-2714-4317","middle_name":"M","organization":"Department of Biochemistry and Molecular Medicine, Universite de Montreal","street":"2900 Edouard Montpetit Blvd","town_or_city":"Montreal","state_or_province":"Quebec","post_or_zip":"H3T 1J4","telephone":null,"fax":null,"first_name":"John","last_name":"Pascal","email":"john.pascal [at] umontreal.ca","country":"Canada","entry":"EMPIAR-12081"}],"status":"REL","deposition_date":"2023-05-10","release_date":"2026-03-30","obsolete_date":null,"update_date":"2026-03-30","corresponding_author":{"author":{"author_orcid":"0000-0003-1405-925X","middle_name":null,"organization":"Department of Biochemistry and Molecular Medicine, Universite de Montreal","street":"2900 Edouard Montpetit Blvd","town_or_city":"Montreal","state_or_province":"Quebec","post_or_zip":"H3T 1J4","first_name":"Aleksandr","last_name":"Sverzhinsky","country":"Canada"}},"authors":[{"author":{"name":"Sverzhinsky A","author_orcid":"0000-0003-1405-925X"}},{"author":{"name":"Pascal JM","author_orcid":"0000-0002-2714-4317"}}],"cross_references":["EMD-48285","EMD-48313"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Sverzhinsky A","author_orcid":"0000-0003-1405-925X"},{"name":"Xue H","author_orcid":null},{"name":"Langelier MF","author_orcid":"0000-0001-9815-1840"},{"name":"Muniz Corrêa MV","author_orcid":null},{"name":"Del Mundo J","author_orcid":null},{"name":"Classen S","author_orcid":"0000-0002-5500-9104"},{"name":"Hammel M","author_orcid":"0000-0002-5610-9289"},{"name":"Rothenberg E","author_orcid":"0000-0002-1382-1380"},{"name":"Pascal JM","author_orcid":"0000-0002-2714-4317"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"PARP1-HPF1 structure and dynamics on nicked DNA suggest a mechanism for acute and localized ADP-ribosylation","volume":"17","country":"","first_page":null,"last_page":null,"year":"2026","language":"English","doi":"10.1038/s41467-026-69375-3","pubmedid":"41698892","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"27.2 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9mi8","9mja"],"entry_doi":"10.6019/EMPIAR-12081"}}