{"EMPIAR-12077":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of respiratory complex I from Paracoccus dentrificans in MSP2N2 nanodiscs","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":16814,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.533,"pixel_height":0.533,"micrographs_file_pattern":"data/sym_link_EMPIAR_Grid1-2","picked_particles_file_pattern":"data/sym_link_EMPIAR_Grid1-2","picked_particles_directory":"data/sym_link_EMPIAR_Grid1-2","details":"Non-gain corrected micrographs were imaged using a Gatan K3 in super-resolution mode and with a GIF BioQuantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 2.4 s exposures captured in 40 frames and a total dose of 46.6 electrons Å-2. Dataset also contains polished (0.533 pixel size) and re-polished (0.534 pixel size) particle stacks and gain reference files.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_UU_00028/1","country":"United Kingdom"},{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_UU_00015/2","country":"United Kingdom"}],"version_history":[],"title":"Single particle dataset of respiratory complex I from Paracoccus dentrificans in MSP2N2 nanodiscs","principal_investigator":[{"author_orcid":"0000-0001-8667-6797","middle_name":null,"organization":"MRC Mitochondrial Biology Unit, University of Cambridge","street":"Keith Peters Building,, Cambridge Biomedical Campus","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0XY","telephone":null,"fax":null,"first_name":"Judy","last_name":"Hirst","email":"jh480 [at] cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-12077"}],"status":"REL","deposition_date":"2024-04-11","release_date":"2024-12-02","obsolete_date":null,"update_date":"2024-12-02","corresponding_author":{"author":{"author_orcid":"0000-0001-8667-6797","middle_name":null,"organization":"MRC Mitochondrial Biology Unit, University of Cambridge","street":"Keith Peters Building, Cambridge Biomedical Campus","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0XY","first_name":"Judy","last_name":"Hirst","country":"United Kingdom"}},"authors":[{"author":{"name":"Ivanov BS","author_orcid":"0000-0002-1039-3894"}},{"author":{"name":"Bridges HR","author_orcid":"0000-0001-6890-6050"}}],"cross_references":["EMD-18325","EMD-18324"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Ivanov BS","author_orcid":"0000-0002-1039-3894"},{"name":"Bridges HR","author_orcid":"0000-0001-6890-6050"},{"name":"Hirst J","author_orcid":"0000-0001-8667-6797"},{"name":"Jarman OD","author_orcid":null}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Structure of the turnover-ready state of an ancestral respiratory complex I","volume":null,"country":"United Kingdom","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.1038/s41467-024-53679-3","pubmedid":"39472559","details":"The micrographs are non-gain corrected, averaged of 40 unaligned frames, with a dose rate of 19.43 e-/Å^2/s over 2.4 s, giving a total dose of 46.6 e-/Å^2.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"6.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8qc1","8qby"],"entry_doi":"10.6019/EMPIAR-12077"}}