{"EMPIAR-12050":{"imagesets":[{"segmentations":[],"name":"Tilt series of ER-ER junctions of HeLa cells in interphase","directory":"data/data/TiltSeries","category":"tilt series","header_format":"PNG","data_format":"TIFF","num_images_or_tilt_series":110,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":4.51,"pixel_height":4.51,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"HeLa cells were instantly frozen using a high-pressure freezing machine (High Pressure Freezer Leica EM ICE equipped with the Coral Life workflow; Leica Microsystems) and freeze-substituted into Agar 100 Epoxy resin (Agar Scientific). As fiducial markers, 15 nm of gold-conjugated Protein A (Cytodiagnostics, AC-15-05-10) was absorbed on both sides of 250 nm sections. Sections were post-stained with 2% UA and lead citrate. Dual axis tilt series were acquired with a Tecnai G2 20 transmission electron microscope operated at 200 kV and equipped with an Eagle 4k HS CCD camera (FEI) by using the Serial EM software. Images were recorded over a -55° to 55° tilt range on average with an angular increment 1°.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Reconstructed tomograms of ER-ER junctions of HeLa cells in interphase","directory":"data/data/Tomograms","category":"reconstructed volumes","header_format":"PNG","data_format":"MRC","num_images_or_tilt_series":110,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":4.51,"pixel_height":4.51,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Vienna Science and Technology Fund (WWTF)","code":"LS19-001","country":"Austria"},{"funding_body":"Austrian Science Fund","code":"P 36743-B","country":"Austria"}],"version_history":[],"title":"Tomograms and the raw 2d tilt series of ER-ER junctions of HeLa cells in interphase","principal_investigator":[{"author_orcid":"0000-0003-3976-0843","middle_name":null,"organization":"Max Perutz Labs","street":null,"town_or_city":"Vienna","state_or_province":null,"post_or_zip":"1030","telephone":null,"fax":null,"first_name":"Shotaro","last_name":"Otsuka","email":"shotaro.otsuka [at] univie.ac.at","country":"Austria","entry":"EMPIAR-12050"}],"status":"REL","deposition_date":"2024-04-19","release_date":"2024-07-04","obsolete_date":null,"update_date":"2024-11-13","corresponding_author":{"author":{"author_orcid":"0000-0003-3976-0843","middle_name":null,"organization":"Max Perutz Labs","street":"Dr. Bohr Gasse 9","town_or_city":"Vienna","state_or_province":null,"post_or_zip":"1030","first_name":"Shotaro","last_name":"Otsuka","country":"Austria"}},"authors":[{"author":{"name":"Bragulat-Teixidor H","author_orcid":"0000-0003-3486-2285"}},{"author":{"name":"Otsuka S","author_orcid":"0000-0003-3976-0843"}}],"cross_references":["EMD-50115"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Bragulat-Teixidor H","author_orcid":"0000-0003-3486-2285"},{"name":"Ishihara K","author_orcid":"0000-0002-8481-8680"},{"name":"Szücs GM","author_orcid":"0000-0003-4842-2862"},{"name":"Otsuka S","author_orcid":"0000-0003-3976-0843"}],"editors":[{"name":" ","author_orcid":null}],"published":true,"j_or_nj_citation":true,"title":"The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after mitosis","volume":null,"country":"","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1038/s44319-024-00175-w","pubmedid":"38877171","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"EMBO reports","journal_abbreviation":"EMBO Rep","issue":null,"preprint":false}],"dataset_size":"304.4 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-12050"}}