{"EMPIAR-12038":{"imagesets":[{"segmentations":[],"name":"Tilt-series of purified SARS-CoV-2 double membrane vesicles formed by nsp3-4","directory":"data","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":4635,"frames_per_image":35,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.56,"pixel_height":1.56,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The tilt-series were acquired using Thermofisher Krios equipped with a Falcon 4i camera and Selectris energy filter, with a slit width of 20 eV. A dose-symmetric scheme (group of 2) was used, with a tilt range of -51° to 51° (or -60° to 60°) at 3° increments and an exposure dose of 3 e-/Å2 per image, and 35 or 41 frames per image. The raw micrographs were motion-corrected in MRC format (5x5 patch using relion_motioncor) and then stacked into individual tilt-series. The tilt-series alignment results (including the transformation matrix *.xf, local transformation file and tilt angle) for RELION4 subtomogram averaging pipeline are provided. The particle_data.star contains the metadata of refined subtomograms. The pixel size of the tilt-series is 1.56 Å and then calibrated into 1.571 Å.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"The University Grants Committee, Research Grants Council (RGC)","code":"#27103023","country":"Hong Kong"}],"version_history":[],"title":"Cryo-ET dataset of purified SARS-CoV-2 double membrane vesicles formed by nsp3-4","principal_investigator":[{"author_orcid":"0000-0001-7268-0306","middle_name":null,"organization":"School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong","street":"21 Sassoon Road, Pokfulam","town_or_city":"Hong Kong","state_or_province":null,"post_or_zip":"0","telephone":"+852 3917 9142","fax":null,"first_name":"Tao","last_name":"Ni","email":"taoni [at] hku.hk","country":"China","entry":"EMPIAR-12038"}],"status":"REL","deposition_date":"2024-04-11","release_date":"2024-07-19","obsolete_date":null,"update_date":"2024-11-19","corresponding_author":{"author":{"author_orcid":"0000-0001-7268-0306","middle_name":null,"organization":"School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong","street":"21 Sassoon Road, Pokfulam","town_or_city":"Hong Kong","state_or_province":null,"post_or_zip":"00000","first_name":"Tao","last_name":"Ni","country":"China"}},"authors":[{"author":{"name":"Huang YX","author_orcid":"0009-0000-5791-4397"}},{"author":{"name":"Ni T","author_orcid":"0000-0001-7268-0306"}}],"cross_references":[],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Ni T","author_orcid":"0000-0001-7268-0306"},{"name":"Huang YX","author_orcid":"0009-0000-5791-4397"},{"name":"Zhong LJ","author_orcid":"0009-0007-8831-001X"}],"editors":[{"name":"Huang YX","author_orcid":"0009-0000-5791-4397"}],"published":false,"j_or_nj_citation":true,"title":"Molecular Architecture of Coronavirus Double Membrane Vesicle Pore Complex","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":"English","doi":"10.1016/s41586-024-07817-y","pubmedid":null,"details":"Sample: Purified SARS-CoV-2 DMV nsp3-4 pore complex\nData collection: The tilt-series were acquired using Thermofisher Krios equipped with a Falcon 4i camera and Selectris energy filter, with a slit width of 20 eV. A dose-symmetric scheme (group of 2) was used, with a tilt range of -51° to 51° (or -60° to 60°) at 3° increments and an exposure dose of 3 e-/Å2 per image.\n\nThe motion-corrected tilt-series and the corresponding IMOD transformation files, tilt angle which are required by RELION4 are uploaded.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"10.3 TB","experiment_type":"ssET","scale":"organism","entry_doi":"10.6019/EMPIAR-12038"}}