{"EMPIAR-11983":{"imagesets":[{"segmentations":[],"name":"Tilt series unaligned raw movies","directory":"data/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1994,"frames_per_image":8,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.368,"pixel_height":1.368,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt series were acquired on a Titan Krios G4i microscope (ThermoFisher Scientific) operated at 300 kV and equipped with a Bioquantum energy filter (Gatan). Movies were collected at a magnification of 64,000x with a K3 Summit direct electron detector (Gatan) operating in super-resolution mode and binned 2x, corresponding to a nominal physical pixel size of 1.368 Å, with 8 frames per movie. Tilts were collected using SerialEM software with a modified dose-symmetric tilt scheme ranging from -54 to +66 degrees, starting from a +6° pretilt and at an interval of 3°. The total dose per tilt series was 100 e-/Å2. A 20 eV energy filter slit was used in the record mode, and the energy filter Zero Loss Peak was refined after each tilt series acquisition.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R01-GM144542","country":"United States"},{"funding_body":"National Science Foundation (NSF, United States)","code":"CAREER-2046778","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"5T32-GM007287","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"1DP2GM150019-01","country":"United States"}],"version_history":[],"title":"Cryo electron tomography of FIB-milled E. coli strain NCM3722 grown to mid-log phase in rich media","principal_investigator":[{"author_orcid":"0000-0002-8858-8907","middle_name":"H","organization":"Department of Biology, Massachusetts Institute of Technology","street":"31 Ames St","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02139","telephone":null,"fax":null,"first_name":"Joseph","last_name":"Davis","email":"jhdavis [at] mit.edu","country":"United States","entry":"EMPIAR-11983"},{"author_orcid":"0000-0002-5934-687X","middle_name":null,"organization":"Life Sciences Institute, University of Michigan","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"Michigan","post_or_zip":"48109","telephone":null,"fax":null,"first_name":"Shyamal","last_name":"Mosalaganti","email":"mosalaga [at] umich.edu","country":"United States","entry":"EMPIAR-11983"}],"status":"REL","deposition_date":"2024-03-20","release_date":"2026-11-02","obsolete_date":null,"update_date":"2026-11-02","corresponding_author":{"author":{"author_orcid":"0000-0002-5934-687X","middle_name":null,"organization":"Life Sciences Institute, University of Michigan","street":"210 Washtenaw Ave","town_or_city":"Ann Arbor","state_or_province":"Michigan","post_or_zip":"48109","first_name":"Shyamal","last_name":"Mosalaganti","country":"United States"}},"authors":[{"author":{"name":"Powell BM","author_orcid":"0000-0003-4228-2977"}},{"author":{"name":"Brant TS","author_orcid":"0000-0001-9609-8273"}},{"author":{"name":"Davis JH","author_orcid":"0000-0002-8858-8907"}},{"author":{"name":"Mosalaganti S","author_orcid":"0000-0002-5934-687X"}}],"cross_references":["EMD-44152"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Powell BM","author_orcid":"0000-0003-4228-2977"},{"name":"Brant TS","author_orcid":"0000-0001-9609-8273"},{"name":"Davis JH","author_orcid":"0000-0002-8858-8907"},{"name":"Mosalaganti S","author_orcid":"0000-0002-5934-687X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Rapid structural analysis of bacterial ribosomes in situ","volume":"8","country":"","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1038/s42003-025-07586-y","pubmedid":"39875527","details":"Raw movies of FIB-milled NCM3722 E.coli imaged in a dose-symmetric tilt series (-54° to +66° in 3° steps starting from +6° pretilt). A total of 53 tilt series were collected at 64,000x magnification with a K3 summit detector operating in super-resolution mode and binned 2x to a nominal physical pixel size of 1.368Å/px. Each movie consists of 8 frames with total dose per movie of 2.5 e-/Å2.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Communications biology","journal_abbreviation":"Commun Biol","issue":"1","preprint":false}],"dataset_size":"46.3 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11983"}}