{"EMPIAR-11961":{"imagesets":[{"segmentations":[],"name":"multi-frame micrographs of the Polymerase module of yeast CPF in complex with Mpe1, the yPIM of Cft2 and the pre-cleaved CYC1 RNA","directory":"data/24032021_JR38-1_JR38-4_PAM-Mpe1-yPIM-42mer_Krios3_K3_105kx_angpix0p86_40TD","category":"micrographs - multiframe","header_format":"MRC","data_format":"TIFF","num_images_or_tilt_series":19524,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.86,"pixel_height":0.86,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_U105192715","country":"United Kingdom"},{"funding_body":"European Research Council (ERC)","code":"725685","country":"United Kingdom"}],"version_history":[],"title":"Polymerase module of yeast CPF in complex with Mpe1, the yPIM of Cft2 and the pre-cleaved CYC1 RNA","principal_investigator":[{"author_orcid":"0000-0003-1815-3710","middle_name":"A","organization":"MRC Laboratory of Molecular Biology","street":"Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Lori","last_name":"Passmore","email":"passmore [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-11961"}],"status":"REL","deposition_date":"2023-12-16","release_date":"2024-06-11","obsolete_date":null,"update_date":"2024-06-11","corresponding_author":{"author":{"author_orcid":"0000-0003-1815-3710","middle_name":"A","organization":"MRC Laboratory of Molecular Biology","street":"Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","first_name":"Lori","last_name":"Passmore","country":"United Kingdom"}},"authors":[{"author":{"name":"Rodríguez-Molina JB","author_orcid":"0000-0003-3305-9722"}},{"author":{"name":"O'Reilly FJ","author_orcid":"0000-0001-9258-0150"}},{"author":{"name":"Fagarasan H","author_orcid":"0000-0003-1775-3271"}},{"author":{"name":"Sheekey E","author_orcid":"0000-0002-1501-550X"}},{"author":{"name":"Maslen S","author_orcid":null}},{"author":{"name":"Skehel JM","author_orcid":"0000-0002-2432-0901"}},{"author":{"name":"Rappsilber J","author_orcid":"0000-0001-5999-1310"}},{"author":{"name":"Passmore LA","author_orcid":"0000-0003-1815-3710"}}],"cross_references":["EMD-14712"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rodríguez-Molina JB","author_orcid":"0000-0003-3305-9722"},{"name":"O'Reilly FJ","author_orcid":"0000-0001-9258-0150"},{"name":"Fagarasan H","author_orcid":"0000-0003-1775-3271"},{"name":"Sheekey E","author_orcid":"0000-0002-1501-550X"},{"name":"Maslen S","author_orcid":null},{"name":"Skehel JM","author_orcid":"0000-0002-2432-0901"},{"name":"Rappsilber J","author_orcid":"0000-0001-5999-1310"},{"name":"Passmore LA","author_orcid":"0000-0003-1815-3710"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF","volume":"82","country":"","first_page":"2490","last_page":"2504.e12","year":"2022","language":"English","doi":"10.1016/j.molcel.2022.04.021","pubmedid":"35584695","details":"For this complex we collected 19,524 movies on Krios III at MRC-LMB with a K3 detector in super-resolution counting mode (bin 2), pixel size of 0.86 Å/pixel, total dose of 40 e-/Å2 with a defocus range of -0.5 μm to -3.1 μm in 0.2 μm steps.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Molecular cell","journal_abbreviation":"Mol Cell","issue":"13","preprint":false}],"dataset_size":"3.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7zgr"],"entry_doi":"10.6019/EMPIAR-11961"}}