{"EMPIAR-11906":{"imagesets":[{"segmentations":[],"name":"Unaligned multiframe micrographs in EER format of the UFM1 ribosome E3 ligase complex bound to the 60S ribosome subunit","directory":"data/Images-Disc1","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":59394,"frames_per_image":819,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.74,"pixel_height":0.74,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dose per physical pixel/s of 6.85 used resulting in a total dose of 33.4 e/Å2. Defocus range of -0.2 to -2. For motion correction, 819 internal frames were grouped by 20 resulting in 42 EER fractions with a dose per frame of 0.8 e/A2. Gain reference file called \"20221115_154105_EER_GainReference.gain\".","image_width":null,"image_height":null},{"segmentations":[],"name":"Aligned micrographs of the UFM1 ribosome E3 ligase complex bound to the 60S ribosome subunit","directory":"data/job005/Raw_data","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":59394,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 20 and dose per frame of 0.8 e/A2. 5, 5 patches were used.","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[{"funding_body":"Wellcome Trust","code":"222531/Z/21/Z","country":"United Kingdom"},{"funding_body":"Wellcome Trust","code":"222372/Z/21/Z","country":"United Kingdom"},{"funding_body":"Biotechnology and Biological Sciences Research Council (BBSRC)","code":"BB/T008172/1","country":"United Kingdom"},{"funding_body":"European Research Council (ERC)","code":"677623","country":"United Kingdom"},{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_UU_00018/3","country":"United Kingdom"},{"funding_body":"Wellcome Trust","code":"221524/Z/20/Z","country":"United Kingdom"}],"version_history":[],"title":"UFM1 ribosome E3 ligase complex bound to the 60S ribosomal subunit","principal_investigator":[{"author_orcid":"0000-0003-0239-5926","middle_name":null,"organization":"Astbury Centre for Structural Molecular Biology, University of Leeds","street":null,"town_or_city":"Leeds","state_or_province":"West Yorkshire","post_or_zip":"LS2 9JT","telephone":null,"fax":null,"first_name":"Elton","last_name":"Zeqiraj","email":"e.zeqiraj [at] leeds.ac.uk","country":"United Kingdom","entry":"EMPIAR-11906"},{"author_orcid":null,"middle_name":null,"organization":"MRC Protein Phosphorylation & Ubiquitylation Unit, University of Dundee","street":"Dow Street","town_or_city":"Dundee","state_or_province":null,"post_or_zip":"DD1 5EH","telephone":null,"fax":null,"first_name":"Yogesh","last_name":"Kulathu","email":"y.kulathu [at] dundee.ac.uk","country":"United Kingdom","entry":"EMPIAR-11906"}],"status":"REL","deposition_date":"2024-01-26","release_date":"2024-07-12","obsolete_date":null,"update_date":"2024-07-12","corresponding_author":{"author":{"author_orcid":"0000-0001-7835-6005","middle_name":null,"organization":"Astbury Centre for Structural Molecular Biology, University of Leeds","street":null,"town_or_city":"Leeds","state_or_province":"West Yorkshire","post_or_zip":"LS2 9JT","first_name":"Linda","last_name":"Makhlouf","country":"United Kingdom"}},"authors":[{"author":{"name":"Makhlouf L","author_orcid":"0000-0001-7835-6005"}}],"cross_references":["EMD-18381","EMD-18382"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Makhlouf L","author_orcid":"0000-0001-7835-6005"},{"name":"Peter JJ","author_orcid":null},{"name":"Magnussen HM","author_orcid":null},{"name":"Thakur R","author_orcid":null},{"name":"Millrine D","author_orcid":null},{"name":"Minshull TC","author_orcid":null},{"name":"Harrison G","author_orcid":null},{"name":"Varghese J","author_orcid":null},{"name":"Lamoliatte F","author_orcid":null},{"name":"Foglizzo M","author_orcid":null},{"name":"Macartney T","author_orcid":null},{"name":"Calabrese AN","author_orcid":null},{"name":"Zeqiraj E","author_orcid":"0000-0003-0239-5926"},{"name":"Kulathu Y","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"The UFM1 E3 ligase recognizes and releases 60S ribosomes from ER translocons","volume":"627","country":"","first_page":"437","last_page":"444","year":"2024","language":"English","doi":"10.1038/s41586-024-07093-w","pubmedid":"38383789","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"8003","preprint":false}],"dataset_size":"22.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8qfc","8qfd"],"entry_doi":"10.6019/EMPIAR-11906"}}