{"EMPIAR-11899":{"imagesets":[{"segmentations":[],"name":"Aligned tilt-series of Dictyostelium discoideum cells","directory":"data","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":213,"frames_per_image":61,"frame_range_min":17,"frame_range_max":61,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":1.223,"pixel_height":1.223,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt-series were aimed to collect 61 tilts, for some fewer tilts were acquired. Tilt-series are sorted according to ascending tilt angle apart from +60 to -60 from lamella pre-tilt (8deg). mdoc files with metadata provided by SerialEM are also provided.\nTilts that were discarded during processing are specified in \"tilt_cleaning.txt\".\nAlignment results from IMOD are provided as _dose-filt.xf files.\nCoordinates to raw ribosome particle picks by template matching are provided in TM_SG_bin6_subtomos_3.1star, with coordinates with bin-factor of 6 corresponding to a pixel size of 7.3 Angstrom. Ribosome particle locations after 3D-classification are provided in Classification_relion_bin6_subtomos_3.1.star, with coordinates with bin-factor of 6 corresponding to a pixel size of 7.3 Angstrom.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Chan Zuckerberg Initiative","code":"2021-234666","country":"United States"}],"version_history":[],"title":"Cryo-ET dataset on lamellae of Dictyostelium discoideum cells [213 tilt-series in MRC format]","principal_investigator":[{"author_orcid":"0000-0002-5457-4478","middle_name":null,"organization":"Max Planck Institute of Biophysics, Department of Molecular Sociology","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hessen","post_or_zip":"60438","telephone":null,"fax":null,"first_name":"Beata","last_name":"Turoňová","email":"beata.turonova [at] biophys.mpg.de","country":"Germany","entry":"EMPIAR-11899"},{"author_orcid":"0000-0002-7397-1321","middle_name":null,"organization":"Max Planck Institute of Biophysics, Department of Molecular Sociology","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hessen","post_or_zip":"60438","telephone":null,"fax":null,"first_name":"Martin","last_name":"Beck","email":"martin.beck [at] biophys.mpg.de","country":"Germany","entry":"EMPIAR-11899"},{"author_orcid":"0000-0001-7768-746X","middle_name":null,"organization":"Max Planck Institute of Biophysics, Department of Theoreotical Biophysics","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hessen","post_or_zip":"60438","telephone":null,"fax":null,"first_name":"Gerhard","last_name":"Hummer","email":"gerhard.hummer [at] biophys.mpg.de","country":"Germany","entry":"EMPIAR-11899"}],"status":"REL","deposition_date":"2024-02-12","release_date":"2024-05-16","obsolete_date":null,"update_date":"2024-05-16","corresponding_author":{"author":{"author_orcid":"0000-0002-5457-4478","middle_name":null,"organization":"Max Planck Institute of Biophysics","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hessen","post_or_zip":"60438","first_name":"Beata","last_name":"Turoňová","country":"Germany"}},"authors":[{"author":{"name":"Tuijtel MW","author_orcid":"0000-0002-0615-572X"}},{"author":{"name":"Kreysing JP","author_orcid":"0000-0002-4770-6313"}},{"author":{"name":"Welsch S","author_orcid":"0000-0002-6049-6664"}},{"author":{"name":"Hummer G","author_orcid":"0000-0001-7768-746X"}},{"author":{"name":"Beck M","author_orcid":"0000-0002-7397-1321"}},{"author":{"name":"Turoňová B","author_orcid":"0000-0002-5457-4478"}},{"author":{"name":"Cruz-León S","author_orcid":"0000-0003-1256-2206"}}],"cross_references":["EMD-19136"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Tuijtel MW","author_orcid":"0000-0002-0615-572X"},{"name":"Cruz-León S","author_orcid":"0000-0003-1256-2206"},{"name":"Kreysing JP","author_orcid":"0000-0002-4770-6313"},{"name":"Welsch S","author_orcid":"0000-0002-6049-6664"},{"name":"Hummer G","author_orcid":"0000-0001-7768-746X"},{"name":"Beck M","author_orcid":"0000-0002-7397-1321"},{"name":"Turoňová B","author_orcid":"0000-0002-5457-4478"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Thinner is not always better: Optimizing cryo-lamellae for subtomogram averaging","volume":"10","country":"","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1126/sciadv.adk6285","pubmedid":"38669330","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"17","preprint":false}],"dataset_size":"382.6 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11899"}}