{"EMPIAR-11857":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 1, Grid A)","directory":"data/Collection_1/Raw_Micrographs_Collection_1_Set_1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":752,"frames_per_image":77,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.42605,"pixel_height":0.42605,"micrographs_file_pattern":"data/Collection_1/Raw_Micrographs_Collection_1_Set_1/20210923_MPS*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned movie files (tif format, 77 frames, not gain corrected).\nCryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. movies were obtained at an exposure rate of 21.13 electrons/Å^2/sec. The total exposure time was 2.717 sec over 77 frames per movie stack.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 1, Grid B)","directory":"data/Collection_1/Raw_Micrographs_Collection_1_Set_2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7258,"frames_per_image":77,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.42605,"pixel_height":0.42605,"micrographs_file_pattern":"data/Collection_1/Raw_Micrographs_Collection_1_Set_2/20210923_MPS*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned movie files (tif format, 77 frames, not gain corrected).\nCryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. movies were obtained at an exposure rate of 21.13 electrons/Å^2/sec. The total exposure time was 2.717 sec over 77 frames per movie stack.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 2, Grid B)","directory":"data/Collection_2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1949,"frames_per_image":77,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.42605,"pixel_height":0.42605,"micrographs_file_pattern":"data/Collection_2/20211220_MPS*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned movie files (tif format, 77 frames, not gain corrected).\nCryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. Movies were obtained at an exposure rate of 20.95 electrons/ Å^2/sec. The total exposure time was 2.717 sec over 77 frames per movie stack.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 3, Grid C)","directory":"data/Collection_3","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":11456,"frames_per_image":79,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.42605,"pixel_height":0.42605,"micrographs_file_pattern":"data/Collection_3/20211220_MPS*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw unaligned movie files (tif format, 79 frames, not gain corrected).\nCryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. Movies were obtained at an exposure rate of 30.71 electrons/Å^2/sec. The total exposure time was 2.008 sec over 79 frames per movie stack.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"K99HL16140601","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"R01GM083118","country":"United States"}],"version_history":[],"title":"β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after addition of GTP","principal_investigator":[{"author_orcid":"0000-0003-3238-3213","middle_name":"M.","organization":"Stanford University","street":null,"town_or_city":"Stanford","state_or_province":"California","post_or_zip":"94305","telephone":null,"fax":null,"first_name":"Makaía","last_name":"Papasergi-Scott","email":"makaia [at] stanford.edu","country":"United States","entry":"EMPIAR-11857"}],"status":"REL","deposition_date":"2024-01-20","release_date":"2024-06-18","obsolete_date":null,"update_date":"2024-06-18","corresponding_author":{"author":{"author_orcid":"0000-0003-0238-7846","middle_name":null,"organization":"Stanford University","street":null,"town_or_city":"Stanford","state_or_province":"California","post_or_zip":"94305","first_name":"Georgios","last_name":"Skiniotis","country":"United States"}},"authors":[{"author":{"name":"Papasergi-Scott MM","author_orcid":"0000-0003-3238-3213"}},{"author":{"name":"Skiniotis G","author_orcid":"0000-0003-0238-7846"}}],"cross_references":["EMD-40004","EMD-42427","EMD-40171","EMD-40123","EMD-42422","EMD-42408","EMD-42412","EMD-40021","EMD-40134","EMD-40126"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Papasergi-Scott MM","author_orcid":"0000-0003-3238-3213"},{"name":"Pérez-Hernández G","author_orcid":null},{"name":"Batebi H","author_orcid":null},{"name":"Gao Y","author_orcid":null},{"name":"Eskici G","author_orcid":null},{"name":"Seven AB","author_orcid":null},{"name":"Panova O","author_orcid":null},{"name":"Hilger D","author_orcid":null},{"name":"Casiraghi C","author_orcid":null},{"name":"He F","author_orcid":null},{"name":"Maul L","author_orcid":null},{"name":"Gmeiner P","author_orcid":null},{"name":"Kobilka BK","author_orcid":null},{"name":"Hildebrand PW","author_orcid":null},{"name":"Skiniotis G","author_orcid":"0000-0003-0238-7846"}],"editors":[{"name":"Skiniotis G","author_orcid":"0000-0003-0238-7846"},{"name":"Papasergi-Scott MM","author_orcid":"0000-0003-3238-3213"}],"published":true,"j_or_nj_citation":true,"title":"Time-resolved cryo-EM of G-protein activation by a GPCR","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1038/s41586-024-07153-1","pubmedid":"38480881","details":"Raw unaligned movie files (tif format, not gain corrected), and gain correction images (mrc format). \nCollected on a Titan Krios equipped with a K3 Summit direct electron detector. The microscope was operated at 300 kV accelerating voltage. Super-resolution pixel size is 0.42605Å.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"12.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8gge","8uo4","8unz","8unl","8unp","8ggu"],"entry_doi":"10.6019/EMPIAR-11857"}}