{"EMPIAR-11844":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of tetrahymena ribozyme.","directory":"data/ThR/exposures","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":18366,"frames_per_image":30,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.82,"pixel_height":0.82,"micrographs_file_pattern":"data/ThR/exposures/GridSquare_*/*_fractions.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"These micrographs were collected with a Titan Krios G3i, 300kV and a K3 detector. Over the collection, the sample was exposed evenly to 57.25 electrons per angstrom squared with a total exposure time of 2.5 seconds. Desired defocuses ranged from -0.5 to -2 nm. The exposures are not gain corrected, the gain reference can be found at data/ThR/exposures/K3-18210041 Gain Ref. x1.m1.mrc.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Extracted particles and orientations for final reconstruction of tetrahymena ribozyme.","directory":"data/ThR/particles","category":"picked particles - single frame - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1181000,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.82,"pixel_height":0.82,"micrographs_file_pattern":"","picked_particles_file_pattern":"data/ThR/particles/data_*/*mrcs","picked_particles_directory":"data/ThR/particles/data_*","details":"Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/ThR/particles/2.2A_cryosparc_P5_J1222_004_particles.cs and data/ThR/particles/2.3A_cryosparc_P5_J1224_004_particles.cs","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"R35GM122579","country":"United States"},{"funding_body":"Howard Hughes Medical Institute (HHMI)","code":"N/A","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"U24GM129541","country":"United States"}],"version_history":[],"title":"Cryo-EM of Tetrahymena ribozyme","principal_investigator":[{"author_orcid":"0000-0002-8910-3078","middle_name":null,"organization":"Department of Bioengineering, James H. Clark Center, Stanford University; CryoEM and Bioimaging Division, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory","street":"318 CAMPUS DR","town_or_city":"Stanford","state_or_province":"CA","post_or_zip":"94305","telephone":null,"fax":null,"first_name":"Wah","last_name":"Chiu","email":"wahc [at] stanford.edu","country":"United States","entry":"EMPIAR-11844"}],"status":"REL","deposition_date":"2023-11-28","release_date":"2025-02-04","obsolete_date":null,"update_date":"2025-02-04","corresponding_author":{"author":{"author_orcid":"0000-0001-7497-0972","middle_name":null,"organization":"Department of Biochemistry, Stanford University; Howard Hughes Medical Institute","street":"279 Campus Dr W","town_or_city":"Stanford","state_or_province":"CA","post_or_zip":"94305","first_name":"Rhiju","last_name":"Das","country":"United States"}},"authors":[{"author":{"name":"Kretsch RC","author_orcid":"0000-0002-6935-518X"}},{"author":{"name":"Li S","author_orcid":"0000-0002-7041-5960"}},{"author":{"name":"Pintilie G","author_orcid":"0000-0002-0848-5335"}},{"author":{"name":"Palo M","author_orcid":"0000-0003-4883-6766"}},{"author":{"name":"Case D","author_orcid":"0000-0003-2314-2346"}},{"author":{"name":"Zhang K","author_orcid":"0000-0003-0414-4776"}},{"author":{"name":"Das R","author_orcid":"0000-0001-7497-0972"}},{"author":{"name":"Chiu W","author_orcid":"0000-0002-8910-3078"}}],"cross_references":["EMD-42498","EMD-42499"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Kretsch RC","author_orcid":null},{"name":"Li S","author_orcid":null},{"name":"Pintilie G","author_orcid":null},{"name":"Palo MZ","author_orcid":null},{"name":"Case DA","author_orcid":null},{"name":"Das R","author_orcid":null},{"name":"Zhang K","author_orcid":null},{"name":"Chiu W","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Complex Water Networks Visualized through 2.2-2.3 Angstrom Cryogenic Electron Microscopy of RNA","volume":null,"country":"","first_page":null,"last_page":null,"year":"2025","language":null,"doi":"10.1101/2025.01.23.634578","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"3.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9cbw","9cby","9cbu","9cbx"],"entry_doi":"10.6019/EMPIAR-11844"}}