{"EMPIAR-11809":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of a group IIC intron (post-exon ligation)","directory":"data/Post_2F_RNP_1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":13740,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.832,"pixel_height":0.832,"micrographs_file_pattern":"data/Post_2F_RNP_1/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of a group IIC intron (post-exon ligation)","directory":"data/Post_2F_RNP_2","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3872,"frames_per_image":48,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.832,"pixel_height":0.832,"micrographs_file_pattern":"data/Post_2F_RNP_2/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"Howard Hughes Medical Institute (HHMI)","code":"HHMI","country":"United States"}],"version_history":[],"title":"CryoEM micrographs of a group II RNP in the post-ligation state","principal_investigator":[{"author_orcid":"0000-0001-9045-8872","middle_name":"Marie","organization":"Molecular, Cellular and Developmental Biology (MCDB), Yale University","street":"260 Whitney Ave","town_or_city":"New Haven","state_or_province":"Connecticut","post_or_zip":"06511","telephone":null,"fax":null,"first_name":"Anna","last_name":"Pyle","email":"anna.pyle [at] yale.edu","country":"United States","entry":"EMPIAR-11809"}],"status":"REL","deposition_date":"2023-10-30","release_date":"2024-07-14","obsolete_date":null,"update_date":"2024-07-14","corresponding_author":{"author":{"author_orcid":"0000-0001-9045-8872","middle_name":"Marie","organization":"Molecular, Cellular and Developmental Biology (MCDB), Yale University","street":"260 Whitney Ave","town_or_city":"New Haven","state_or_province":"Connecticut","post_or_zip":"06511","first_name":"Anna","last_name":"Pyle","country":"United States"}},"authors":[{"author":{"name":"Xu L","author_orcid":"0000-0002-9057-0829"}},{"author":{"name":"Liu T","author_orcid":"0009-0006-7764-7402"}},{"author":{"name":"Chung K","author_orcid":"0000-0003-0402-6833"}}],"cross_references":["EMD-40987"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Xu L","author_orcid":"0000-0002-9057-0829"},{"name":"Liu T","author_orcid":"0009-0006-7764-7402"},{"name":"Chung K","author_orcid":"0000-0003-0402-6833"},{"name":"Pyle AM","author_orcid":"0000-0001-9045-8872"}],"editors":[{"name":"Chung K","author_orcid":"0000-0003-0402-6833"}],"published":true,"j_or_nj_citation":true,"title":"Structural insights into intron catalysis and dynamics during splicing","volume":null,"country":"United States","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1038/s41586-023-06746-6","pubmedid":"37993708","details":"For the Chameleon dataset (13 740 micrographs), a nominal magnification of ×105,000 and a defocus range of −1.0 to −2.5 μm was used, giving an effective pixel size of 0.832 Å at the specimen level. Each micrograph was dose-fractionated to 50 frames with a total exposure time of 1.5 s and a frame exposure time of 0.03 s, resulting in a total dose of 50.5 e−/Å2. For the Vitrobot dataset (3 872 micrographs), a nominal magnification of ×105,000 and a defocus range of −1.0 to −2.0 μm was used, giving an effective pixel size of 0.832 Å at the specimen level. Each micrograph was dose-fractionated to 48 frames with a total exposure time of 1.92 s and a frame exposure time of 0.04 s, resulting in a total dose of 50 e−/Å2.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":null,"preprint":false}],"dataset_size":"5.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8t2t"],"entry_doi":"10.6019/EMPIAR-11809"}}