{"EMPIAR-11803":{"imagesets":[{"segmentations":[],"name":"Unaligned multiframe micrographs","directory":"data/rawdata","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":22411,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Multiframe micrographs in TIFF format that were used as the basis for processing and determining a cryo-EM map for the UMP-i structure (EMD-40699, PDB-8SQ9).","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"AI161278","country":"United States"}],"version_history":[],"title":"Structural and functional insights into the enzymatic plasticity of the SARS-CoV-2 NiRAN domain","principal_investigator":[{"author_orcid":"0000-0002-1332-128X","middle_name":null,"organization":"Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Elizabeth","last_name":"Campbell","email":"campbee [at] rockefeller.edu","country":"United States","entry":"EMPIAR-11803"},{"author_orcid":"0000-0002-8241-3153","middle_name":null,"organization":"Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Seth","last_name":"Darst","email":"darst [at] rockefeller.edu","country":"United States","entry":"EMPIAR-11803"}],"status":"REL","deposition_date":"2023-11-16","release_date":"2025-07-03","obsolete_date":null,"update_date":"2025-07-03","corresponding_author":{"author":{"author_orcid":"0000-0002-1332-128X","middle_name":null,"organization":"Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","first_name":"Elizabeth","last_name":"Campbell","country":"United States"}},"authors":[{"author":{"name":"Campbell EA","author_orcid":"0000-0002-1332-128X"}},{"author":{"name":"Small GI","author_orcid":"0000-0002-3506-5256"}},{"author":{"name":"Darst SA","author_orcid":"0000-0002-8241-3153"}}],"cross_references":["EMD-40699"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Small GI","author_orcid":"0000-0002-3506-5256"},{"name":"Fedorova O","author_orcid":null},{"name":"Olinares PDB","author_orcid":null},{"name":"Chandanani J","author_orcid":null},{"name":"Banerjee A","author_orcid":null},{"name":"Choi YJ","author_orcid":null},{"name":"Molina H","author_orcid":null},{"name":"Chait BT","author_orcid":null},{"name":"Darst SA","author_orcid":"0000-0002-8241-3153"},{"name":"Campbell EA","author_orcid":"0000-0002-1332-128X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural and functional insights into the enzymatic plasticity of the SARS-CoV-2 NiRAN domain","volume":"83","country":"United States","first_page":"3921","last_page":"3930.e7","year":"2023","language":"English","doi":"10.1016/j.molcel.2023.10.001","pubmedid":"37890482","details":"Grids were imaged using a 300 kV Titan Krios (Thermo Fisher Scientific) equipped with a GIF BioQuantum and K3 camera (Gatan). Movies were collected with Leginon60 with a pixel size of 1.0825 Å per px (micrograph dimensions of 5,760 × 4,092 px) over a defocus range of 0.7 to 2.5 μm with a 20 eV energy filter slit. Videos were recorded in counting mode (native K3 camera binning 2) with ∼25 e−/ Å2/s exposure rate in dose-fractionation mode with intermediate frames recorded every 50 ms over a 2 s exposure (40 frames per micrograph) to give an electron exposure of ∼51 e−/Å2. Dose-fractionated videos were gain-normalized, drift-corrected, summed and dose-weighted using MotionCor2.48 The CTF was estimated for each summed image using the Patch CTF module in cryoSPARC v.3.2.0.46 Particles were picked and extracted from the dose-weighted images with box size of 256 px using cryoSPARC blob picker and particle extraction. The entire dataset consisted of 21,859 motion-corrected images with 10,257,549 particles.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Molecular cell","journal_abbreviation":"Mol Cell","issue":"21","preprint":false}],"dataset_size":"5.3 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8sq9"],"entry_doi":"10.6019/EMPIAR-11803"}}