{"EMPIAR-11797":{"imagesets":[{"segmentations":[],"name":"Unaligned multiframe movies of RNA polymerase collected at various tilts","directory":"data/movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1146,"frames_per_image":51,"frame_range_min":1,"frame_range_max":50,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/micrographs/T*0/*mrc","picked_particles_file_pattern":"data/stack/RNAP-T*0-Polished.star","picked_particles_directory":"data/stack","details":"Movies collected using Titan Krios and K3 quantum with energy filter. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders. 50 frames were used, pixel size is 0.830 Å/pixel, total dose of 34.2 e-/Å2 (0 degrees) and 38.8 e-/Å2 (60 degrees). Gain reference is 22nov04c_04153642_01_4092x5760_norm_0.mrc.","image_width":"4092","image_height":"5760"},{"segmentations":[],"name":"Aligned and dose-weighted micrographs of RNA polymerase collected at various tilts","directory":"data/micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1146,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/micrographs/T*0/*mrc","picked_particles_file_pattern":"data/stack/RNAP-T*0-Polished.star","picked_particles_directory":"data/stack","details":"Aligned and dose weighted using UCSF MotionCor2. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders.","image_width":"4092","image_height":"5760"},{"segmentations":[],"name":"Polished and equalized particles of RNA polymerase collected at various tilts","directory":"data/stack","category":"picked particles - multiframe - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":2,"frames_per_image":72000,"frame_range_min":1,"frame_range_max":72000,"voxel_type":"32 BIT FLOAT","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/micrographs/T*0/*mrc","picked_particles_file_pattern":"data/stack/RNAP-T*0-Polished.star","picked_particles_directory":"data/stack","details":"Final particle stack after particle polishing using Relion. The particles are equalized so that there are same number of particles per tilt angle with similar defocus distribution.","image_width":"312","image_height":"312"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"U54 AI170855","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"U01 AI136680","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"GM148476","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"GM082946","country":"United States"},{"funding_body":"National Science Foundation (NSF, United States)","code":"MCB 2048095","country":"United States"},{"funding_body":"Other private","code":"Hearst Foundations Developmental Chair","country":"United States"},{"funding_body":"Other private","code":"Margaret T. Morris Foundation","country":"United States"},{"funding_body":"National Research Foundation (NRF, Singapore)","code":"NRF Fellowship A-8001346-00-00","country":"Singapore"},{"funding_body":"Other government","code":"National University of Singapore (PYP Fellowship A-0008405-00-00, A-0008405-01-00)","country":"Singapore"},{"funding_body":"Ministry of Education (MoE, Singapore)","code":"MOE AcRF Tier 1 A-8000037-00-00","country":"Singapore"},{"funding_body":"Ministry of Education (MoE, Singapore)","code":"MOE AcRF Tier 2 A-6100427-01-00","country":"Singapore"},{"funding_body":"Other government","code":"Agency for Science, Technology and Research, Singapore","country":"Singapore"},{"funding_body":"Damon Runyon Cancer Research Foundation","code":"Nadia’s Gift Foundation Innovator Award DRR-65-21","country":"United States"},{"funding_body":"American Cancer Society","code":"PF-21-075-01-CCB","country":"United States"},{"funding_body":"Other government","code":"Cancer Center Support Grant P30 CA01495t","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)","code":"GM103310","country":"United States"},{"funding_body":"Simons Foundation","code":"SF349247","country":"United States"},{"funding_body":"Other government","code":"NYSTAR","country":"United States"},{"funding_body":"Other government","code":"S10 OD032467","country":"United States"},{"funding_body":"National Science Foundation (NSF, United States)","code":"MCB-1939086","country":"United States"}],"version_history":[],"title":"Single-Particle Cryo-EM of RNA Polymerase at Various Tilts","principal_investigator":[{"author_orcid":"0000-0001-6656-6320","middle_name":null,"organization":"National University of Singapore","street":"14 Science Drive 4 Singapore","town_or_city":"Singapore","state_or_province":null,"post_or_zip":"117543","telephone":"+65 6516 7835","fax":null,"first_name":"Yong Zi","last_name":"Tan","email":"yztan [at] nus.edu.sg","country":"Singapore","entry":"EMPIAR-11797"},{"author_orcid":"0000-0002-8124-7472","middle_name":null,"organization":"Salk Institute","street":"10010 N. Torrey Pines Rd.","town_or_city":"San Diego","state_or_province":"California","post_or_zip":"92037","telephone":"+1 858-453-4100 x1155","fax":null,"first_name":"Dmitry","last_name":"Lyumkis","email":"dlyumkis [at] salk.edu","country":"United States","entry":"EMPIAR-11797"}],"status":"REL","deposition_date":"2023-11-27","release_date":"2024-03-26","obsolete_date":null,"update_date":"2024-03-26","corresponding_author":{"author":{"author_orcid":"0000-0001-6656-6320","middle_name":null,"organization":"National University of Singapore","street":"14 Science Drive 4 Singapore","town_or_city":"Singapore","state_or_province":null,"post_or_zip":"117543","first_name":"Yong Zi","last_name":"Tan","country":"Singapore"}},"authors":[{"author":{"name":"Aiyer S","author_orcid":"0000-0001-6436-0521"}},{"author":{"name":"Baldwin PR","author_orcid":"0000-0002-4202-4129"}},{"author":{"name":"Tan SM","author_orcid":"0000-0002-0231-3620"}},{"author":{"name":"Shan Z","author_orcid":"0000-0003-4983-3575"}},{"author":{"name":"Oh J","author_orcid":"0000-0002-7625-2779"}},{"author":{"name":"Mehrani A","author_orcid":"0000-0001-5239-955X"}},{"author":{"name":"Bowman ME","author_orcid":null}},{"author":{"name":"Louie G","author_orcid":"0000-0002-8043-4348"}},{"author":{"name":"Passos DO","author_orcid":"0000-0003-1333-9102"}},{"author":{"name":"Đorđević-Marquardt S","author_orcid":"0000-0003-0170-5304"}},{"author":{"name":"Mietzsch M","author_orcid":"0000-0001-8957-3363"}},{"author":{"name":"Hull JA","author_orcid":"0000-0003-0847-385X"}},{"author":{"name":"Hoshika S","author_orcid":"0000-0001-9128-1183"}},{"author":{"name":"Barad BA","author_orcid":"0000-0002-1016-862X"}},{"author":{"name":"Grotjahn DA","author_orcid":"0000-0001-5908-7882"}},{"author":{"name":"McKenna R","author_orcid":"0000-0001-5129-7850"}},{"author":{"name":"Agbandje-McKenna M","author_orcid":"0000-0003-0565-5578"}},{"author":{"name":"Benner 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