{"EMPIAR-11795":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx.","directory":"data/set1_0tilt/movies","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2198,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.815,"pixel_height":0.815,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. 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Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames). 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Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames). Stage was tilted at 25 degrees.","image_width":"3838","image_height":"3710"},{"segmentations":[],"name":"Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx at 25 degree tilt","directory":"data/set4_25tilt/micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2103,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.815,"pixel_height":0.815,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames). Stage was tilted at 25 degrees.","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R01-GM099813","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"R35-GM138206","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"T32-GM008326","country":"United States"},{"funding_body":"National Aeronautic Space Administration (NASA, United States)","code":"ENIGMA","country":"United States"},{"funding_body":"Kinship Foundation","code":"Searle Scholars Program","country":"United States"}],"version_history":[],"title":"CryoEM movies of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx","principal_investigator":[{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry, University of California, San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","telephone":"7606134160","fax":null,"first_name":"Mark","last_name":"Herzik Jr","email":"bcook [at] ucsd.edu","country":"United States","entry":"EMPIAR-11795"},{"author_orcid":"0000-0002-4733-6500","middle_name":null,"organization":"Department of Chemistry and Biochemistry, University of California, San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","telephone":"858 534 4862","fax":"858 534 6157","first_name":"Faik Akif","last_name":"Tezcan","email":"tezcan [at] ucsd.edu","country":"United States","entry":"EMPIAR-11795"}],"status":"REL","deposition_date":"2023-08-12","release_date":"2024-01-15","obsolete_date":null,"update_date":"2024-01-15","corresponding_author":{"author":{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry, University of California, San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","first_name":"Mark","last_name":"Herzik Jr","country":"United States"}},"authors":[{"author":{"name":"Rutledge HL","author_orcid":"0000-0002-8672-2797"}},{"author":{"name":"Cook BD","author_orcid":"0000-0002-5777-3464"}},{"author":{"name":"Nguyen HPM","author_orcid":"0000-0002-1614-7424"}},{"author":{"name":"Tezcan FA","author_orcid":"0000-0002-4733-6500"}},{"author":{"name":"Herzik Jr MA","author_orcid":"0000-0001-6653-6682"}}],"cross_references":["EMD-26764"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rutledge HL","author_orcid":"0000-0002-8672-2797"},{"name":"Cook BD","author_orcid":"0000-0002-5777-3464"},{"name":"Nguyen HPM","author_orcid":"0000-0002-1614-7424"},{"name":"Herzik Jr MA","author_orcid":"0000-0001-6653-6682"},{"name":"Tezcan FA","author_orcid":"0000-0002-4733-6500"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structures of the nitrogenase complex prepared under catalytic turnover conditions","volume":"377","country":"","first_page":"865","last_page":"869","year":"2022","language":"English","doi":"10.1126/science.abq7641","pubmedid":"35901182","details":"CryoEM movies of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions in the presence of the inhibitor of berrylium fluoride (BeFx). Data were collected on a G3 Titan Krios (300 kV) at a nominal magnification of 165,000x (0.815 Å/pixel) in EFTEM mode on a Gatan K2 detector post a Quantum energy filter (20 eV slit) and a cumulative electron exposure of ~65 electrons/Å2 (50 frames) recorded in counting mode. Data were collected automatically using EPU with aberration free image shift using a defocus range of -0.5 – -2.5 μm. Four separate data sets were collected using 0˚, 15˚ or 25˚ specimen tilt.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":"6608","preprint":false}],"dataset_size":"5.8 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7uta"],"entry_doi":"10.6019/EMPIAR-11795"}}