{"EMPIAR-11789":{"imagesets":[{"segmentations":[],"name":"Unaligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions","directory":"data/Movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":14835,"frames_per_image":66,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.835,"pixel_height":0.835,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions","directory":"data/Micrographs","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":14835,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.835,"pixel_height":0.835,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Gain file for motion correction of unaligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions","directory":"data/Gain","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.835,"pixel_height":0.835,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain file required for motion correction of unaligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions. Note: The pixel sized originally supplied (0.86) was corrected to 0.835 angstroms per pixel.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R01-GM099813","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"R35-GM138206","country":"United States"},{"funding_body":"National Institutes of Health (NIH)","code":"T32-GM008326","country":"United States"},{"funding_body":"National Aeronautic Space Administration (NASA, United States)","code":"ENIGMA","country":"United States"},{"funding_body":"Kinship Foundation","code":"Searle Scholars Program","country":"United States"}],"version_history":[],"title":"CryoEM movies of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions","principal_investigator":[{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry, University of California, San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","telephone":"858 246 5875","fax":null,"first_name":"Mark","last_name":"Herzik Jr","email":"mherzik [at] ucsd.edu","country":"United States","entry":"EMPIAR-11789"},{"author_orcid":"0000-0002-4733-6500","middle_name":null,"organization":"Department of Chemistry and Biochemistry, University of California, San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","telephone":"858 534 4862","fax":"858 534 6157","first_name":"Faik Akif","last_name":"Tezcan","email":"tezcan [at] ucsd.edu","country":"United States","entry":"EMPIAR-11789"}],"status":"REL","deposition_date":"2023-08-08","release_date":"2024-06-17","obsolete_date":null,"update_date":"2024-06-17","corresponding_author":{"author":{"author_orcid":"0000-0001-6653-6682","middle_name":"A","organization":"Department of Chemistry and Biochemistry, University of California, San Diego","street":"9500 Gilman Drive","town_or_city":"La Jolla","state_or_province":"California","post_or_zip":"92093","first_name":"Mark","last_name":"Herzik Jr","country":"United States"}},"authors":[{"author":{"name":"Rutledge HL","author_orcid":"0000-0002-8672-2797"}},{"author":{"name":"Cook BD","author_orcid":"0000-0002-5777-3464"}},{"author":{"name":"Nguyen HPM","author_orcid":"0000-0002-1614-7424"}},{"author":{"name":"Tezcan FA","author_orcid":"0000-0002-4733-6500"}},{"author":{"name":"Herzik Jr MA","author_orcid":"0000-0001-6653-6682"}}],"cross_references":["EMD-26759","EMD-26760","EMD-26762","EMD-26763","EMD-26761","EMD-26758"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rutledge HL","author_orcid":"0000-0002-8672-2797"},{"name":"Cook BD","author_orcid":"0000-0002-5777-3464"},{"name":"Nguyen HPM","author_orcid":"0000-0002-1614-7424"},{"name":"Herzik MA","author_orcid":"0000-0001-6653-6682"},{"name":"Tezcan FA","author_orcid":"0000-0002-4733-6500"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structures of the nitrogenase complex prepared under catalytic turnover conditions","volume":"377","country":"","first_page":"865","last_page":"869","year":"2022","language":"English","doi":"10.1126/science.abq7641","pubmedid":"35901182","details":"CryoEM movies of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions. Data were collected on a G3i Titan Krios (300 kV) at a nominal magnification of 135,000x (0.835 Å/pixel) on a K3 detector post a BioQuantum energy filter (20 eV slit) and a cumulative exposure of ~65 electrons/Å2 (66 frames) recorded in counting mode. Data were collected automatically using EPU with aberration free image shift using a defocus range of -0.5 – -2.5 μm.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":"6608","preprint":false}],"dataset_size":"4.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7ut8","7ut9"],"entry_doi":"10.6019/EMPIAR-11789"}}