{"EMPIAR-11779":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of SpCas9 bound to 6 nucleotide complementary DNA substrate","directory":"data/20210301","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5988,"frames_per_image":36,"frame_range_min":1,"frame_range_max":36,"voxel_type":"UNSIGNED BYTE","pixel_width":0.325,"pixel_height":0.325,"micrographs_file_pattern":"data/20210301/*_fractions.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame movies with each containing 37 frames per movie collected at 300 kV in super-resolution counting mode. Total accumulated electron exposure on the specimen level corresponds to 64.2 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Gain reference","directory":"data/20210301/GainRef_01_03_2021","category":"micrographs - single frame","header_format":"DM4","data_format":"DM4","num_images_or_tilt_series":1,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.325,"pixel_height":0.325,"micrographs_file_pattern":"data/20210301/GainRef_01_03_2021/K3-18290183 Gain Ref. x1.m1.dm4","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Gain reference","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"Swiss National Science Foundation","code":"31003A_182567","country":"Switzerland"}],"version_history":[],"title":"SpCas9 bound to 6 nucleotide complementary DNA substrate","principal_investigator":[{"author_orcid":"0000-0002-7601-210X","middle_name":null,"organization":"Department of Biochemistry, University of Zurich","street":"Winterthurerstrasse 190","town_or_city":"Zurich","state_or_province":"ZH","post_or_zip":"8057","telephone":"41446355572","fax":null,"first_name":"Martin","last_name":"Jinek","email":"jinek [at] bioc.uzh.ch","country":"Switzerland","entry":"EMPIAR-11779"}],"status":"REL","deposition_date":"2023-11-10","release_date":"2024-01-23","obsolete_date":null,"update_date":"2024-01-23","corresponding_author":{"author":{"author_orcid":"0000-0002-7601-210X","middle_name":null,"organization":"Department of Biochemistry, University of Zurich","street":"Winterthurerstrasse 190","town_or_city":"Zurich","state_or_province":"ZH","post_or_zip":"8057","first_name":"Martin","last_name":"Jinek","country":"Switzerland"}},"authors":[{"author":{"name":"Pacesa M","author_orcid":"0000-0003-1560-5769"}},{"author":{"name":"Loeff L","author_orcid":"0000-0002-1805-7844"}},{"author":{"name":"Querques I","author_orcid":"0000-0003-1113-2773"}},{"author":{"name":"Muckenfuss LM","author_orcid":"0000-0003-3558-7211"}},{"author":{"name":"Sawicka M","author_orcid":"0000-0003-4589-4290"}},{"author":{"name":"Jinek M","author_orcid":"0000-0002-7601-210X"}}],"cross_references":["EMD-14493"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Pacesa M","author_orcid":"0000-0003-1560-5769"},{"name":"Loeff L","author_orcid":"0000-0002-1805-7844"},{"name":"Querques I","author_orcid":"0000-0003-1113-2773"},{"name":"Muckenfuss LM","author_orcid":"0000-0003-3558-7211"},{"name":"Sawicka M","author_orcid":"0000-0003-4589-4290"},{"name":"Jinek M","author_orcid":"0000-0002-7601-210X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"R-loop formation and conformational activation mechanisms of Cas9","volume":"609","country":"","first_page":"191","last_page":"196","year":"2022","language":"English","doi":"10.1038/s41586-022-05114-0","pubmedid":"36002571","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"7925","preprint":false}],"dataset_size":"1.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7z4c"],"entry_doi":"10.6019/EMPIAR-11779"}}