{"EMPIAR-11765":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of integrin a4b7 bound to MadCAM-1","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3223,"frames_per_image":100,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.561,"pixel_height":0.561,"micrographs_file_pattern":"data/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"α4β7 integrin bound to MadCAM-1: 3223 unaligned uncorrected movies collected in two different datasets (803 + 2420) were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7).  The movies contain 100 frames and the super resolution pixel size is 0.561 Å/px. The total dose per movie is ~50 e/Å^2. The gain reference is also included for each dataset in .dm4 format; 20220516_K3GainRefx1m3.dm4 is used to reference movies 20220517**.tif and 20220522_K3GainRefx1m3.dm4 is used to reference movies 20220525**.tif.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dose-weighted aligned micrographs of integrin a4b7 bound to MadCAM-1","directory":"data","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3223,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.122,"pixel_height":1.122,"micrographs_file_pattern":"data/*.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"α4β7 integrin bound to MadCAM-1: 3223 unaligned uncorrected movies collected over 2 datasets (803 + 2420) were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dose-weighted aligned particle stacks of clasped apo-integrin a4b7 in the compact state","directory":"data","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":188443,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.122,"pixel_height":1.122,"micrographs_file_pattern":"data/*.mrcs","picked_particles_file_pattern":"data/*.star","picked_particles_directory":"data/","details":"Stacks of 188443 particles selected after sequential 2D and 3D classification contributing to final reconstruction of α4β7 integrin bound to MadCAM-1. Unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.","image_width":"364","image_height":"364"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R35GM147414","country":"United States"}],"version_history":[],"title":"Single-particle cryo electron microscopy of Integrin a4b7 bound to MadCAM-1","principal_investigator":[{"author_orcid":"0000-0003-3375-8937","middle_name":null,"organization":"Basic Sciences Division, Fred Hutchinson Cancer Center","street":"1100 Fairview Ave. N.","town_or_city":"Seattle","state_or_province":"Washington","post_or_zip":"98109","telephone":null,"fax":null,"first_name":"Jeremy","last_name":"Hollis","email":"jhollis [at] fredhutch.org","country":"United States","entry":"EMPIAR-11765"}],"status":"REL","deposition_date":"2023-11-03","release_date":"2025-11-21","obsolete_date":null,"update_date":"2025-11-21","corresponding_author":{"author":{"author_orcid":"0000-0003-1909-5751","middle_name":null,"organization":"Basic Sciences Division, Fred Hutchinson Cancer Center","street":"1100 Fairview Ave. N.","town_or_city":"Seattle","state_or_province":"Washington","post_or_zip":"98109","first_name":"Melody","last_name":"Campbell","country":"United States"}},"authors":[{"author":{"name":"Hollis JA","author_orcid":"0000-0003-3375-8937"}},{"author":{"name":"Campbell MG","author_orcid":"0000-0003-1909-5751"}}],"cross_references":["EMD-71399"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Hollis JA","author_orcid":"0000-0003-3375-8937"},{"name":"Malik HS","author_orcid":"0000-0001-6005-0016"},{"name":"Campbell MG","author_orcid":"0000-0003-1909-5751"},{"name":"Chan MC","author_orcid":"0000-0002-9826-1983"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Molecular exaptation by the integrin αI domain","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.1126/sciadv.adx9567","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"3.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9p95"],"entry_doi":"10.6019/EMPIAR-11765"}}