{"EMPIAR-11764":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of clasped apo-integrin a4b7 in the compact state","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1363,"frames_per_image":100,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":0.561,"pixel_height":0.561,"micrographs_file_pattern":"data/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"α4β7 integrin in the apo state: 1363 unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7).  The movies contain 100 frames and the super resolution pixel size is 0.561 Å/px. The total dose per movie is ~50 e/Å^2. The gain reference is also included as a .dm4 file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dose-weighted aligned micrographs of clasped apo-integrin a4b7 in the compact state","directory":"data","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1363,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.122,"pixel_height":1.122,"micrographs_file_pattern":"data/*.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"α4β7 integrin in the apo state: 1363 unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dose-weighted aligned particle stacks of clasped apo-integrin a4b7 in the compact state","directory":"data","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":239117,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.122,"pixel_height":1.122,"micrographs_file_pattern":"data/*.mrcs","picked_particles_file_pattern":"data/23jan11b_cs_P30_J481_particles_swapxy.star","picked_particles_directory":"data/","details":"Stacks of 239117 particles selected after sequential 2D and 3D classification contributing to final reconstruction of α4β7 integrin in the apo state. Unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.","image_width":"328","image_height":"328"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R35GM147414","country":"United States"}],"version_history":[],"title":"Single-particle cryo electron microscopy of apo-Integrin a4b7 in the compact state","principal_investigator":[{"author_orcid":"0000-0003-3375-8937","middle_name":null,"organization":"Basic Sciences Division, Fred Hutchinson Cancer Center","street":"1100 Fairview Ave. N.","town_or_city":"Seattle","state_or_province":"Washington","post_or_zip":"98109","telephone":null,"fax":null,"first_name":"Jeremy","last_name":"Hollis","email":"jhollis [at] fredhutch.org","country":"United States","entry":"EMPIAR-11764"}],"status":"REL","deposition_date":"2023-11-03","release_date":"2025-07-16","obsolete_date":null,"update_date":"2025-11-05","corresponding_author":{"author":{"author_orcid":"0000-0003-1909-5751","middle_name":null,"organization":"Basic Sciences Division, Fred Hutchinson Cancer Center","street":"1100 Fairview Ave. N.","town_or_city":"Seattle","state_or_province":"Washington","post_or_zip":"98109","first_name":"Melody","last_name":"Campbell","country":"United States"}},"authors":[{"author":{"name":"Hollis JA","author_orcid":"0000-0003-3375-8937"}},{"author":{"name":"Campbell MG","author_orcid":"0000-0003-1909-5751"}}],"cross_references":["EMD-71400"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Hollis JA","author_orcid":"0000-0003-3375-8937"},{"name":"Chan MC","author_orcid":"0000-0002-9826-1983"},{"name":"Malik HS","author_orcid":"0000-0001-6005-0016"},{"name":"Campbell MG","author_orcid":"0000-0003-1909-5751"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Molecular exaptation by the integrin αI domain","volume":"11","country":"","first_page":null,"last_page":null,"year":"2025","language":"English","doi":"10.1126/sciadv.adx9567","pubmedid":"40929264","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"37","preprint":false}],"dataset_size":"1.4 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["9p96"],"entry_doi":"10.6019/EMPIAR-11764"}}