{"EMPIAR-11747":{"imagesets":[{"segmentations":[],"name":"Denoised bin4 tomograms","directory":"data/bin4_cryocare","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7,"frames_per_image":512,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":8.572,"pixel_height":8.572,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms denoised using cryo-CARE trained on each individual tomogram reconstructed from odd/even frames.","image_width":"1024","image_height":"1440"},{"segmentations":[],"name":"IMOD bin4 tomograms","directory":"data/bin4_imod","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7,"frames_per_image":512,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":8.572,"pixel_height":8.572,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"IMOD reconstruction at bin4 using weighted backprojection without CTF correction. IMOD reconstruction files (.xf and .tlt) are provided in the imod_files/ directory.","image_width":"1024","image_height":"1440"},{"segmentations":[],"name":"Tilt series assembled from motion-corrected and averaged frames","directory":"data/raw_stacks","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7,"frames_per_image":53,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":2.143,"pixel_height":2.143,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Frames aligned with MotionCor2\nNote: Frames per image varies","image_width":"5760","image_height":"4096"},{"segmentations":[],"name":"Raw frames","directory":"data/raw_frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":327,"frames_per_image":5,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.071,"pixel_height":1.071,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Movies acquired with a K3 detector in super-resolution mode using SerialEM. Gain reference and defects file are provided in the gainref/ directory. Metadata from the acquisition can be found in the mdocs/ directory.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"European Research Council (ERC)","code":"M822.00045","country":"Switzerland"}],"version_history":[],"title":"In situ cryo-electron tomography of the T. pseudonana diatom PyShell","principal_investigator":[{"author_orcid":"0000-0002-0941-4387","middle_name":"D.","organization":"Biozentrum, University of Basel","street":null,"town_or_city":"Basel","state_or_province":null,"post_or_zip":"4056","telephone":null,"fax":null,"first_name":"Benjamin","last_name":"Engel","email":"ben.engel [at] unibas.ch","country":"Switzerland","entry":"EMPIAR-11747"}],"status":"REL","deposition_date":"2023-10-20","release_date":"2024-10-08","obsolete_date":null,"update_date":"2024-10-08","corresponding_author":{"author":{"author_orcid":"0000-0003-4247-4303","middle_name":"Diogo","organization":"Biozentrum, University of Basel","street":null,"town_or_city":"Basel","state_or_province":null,"post_or_zip":"4056","first_name":"Ricardo","last_name":"Righetto","country":"Switzerland"}},"authors":[{"author":{"name":"Demulder M","author_orcid":"0000-0002-6015-850X"}},{"author":{"name":"Righetto RD","author_orcid":"0000-0003-4247-4303"}},{"author":{"name":"Wietrzynski W","author_orcid":"0000-0001-8898-2392"}},{"author":{"name":"Lamm L","author_orcid":null}},{"author":{"name":"Engel BD","author_orcid":"0000-0002-0941-4387"}}],"cross_references":["EMD-18709"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Shimakawa G","author_orcid":null},{"name":"Demulder M","author_orcid":"0000-0002-6015-850X"},{"name":"Flori S","author_orcid":null},{"name":"Kawamoto A","author_orcid":null},{"name":"Tsuji Y","author_orcid":null},{"name":"Nawaly H","author_orcid":null},{"name":"Tanaka A","author_orcid":null},{"name":"Tohda R","author_orcid":null},{"name":"Ota T","author_orcid":null},{"name":"Matsui H","author_orcid":null},{"name":"Morishima N","author_orcid":null},{"name":"Okubo R","author_orcid":null},{"name":"Wietrzynski W","author_orcid":"0000-0001-8898-2392"},{"name":"Lamm L","author_orcid":null},{"name":"Righetto RD","author_orcid":"0000-0003-4247-4303"},{"name":"Uwizeye C","author_orcid":null},{"name":"Gallet B","author_orcid":null},{"name":"Jouneau PH","author_orcid":null},{"name":"Gerle C","author_orcid":null},{"name":"Kurisu G","author_orcid":null},{"name":"Finazzi G","author_orcid":null},{"name":"Engel BD","author_orcid":"0000-0002-0941-4387"},{"name":"Matsuda Y","author_orcid":null}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Diatom pyrenoids are encased in a protein shell that enables efficient CO2 fixation","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.1016/j.cell.2024.09.013","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"81.8 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11747"}}