{"EMPIAR-11742":{"imagesets":[{"segmentations":[],"name":"Unaligned mutliframe non-gain-normalized EER movies of SpNOX with NADPH collected on a Krios G4","directory":"data/spNOX_NADPHbound_aerobic","category":"micrographs - multiframe","header_format":"EER","data_format":"EER","num_images_or_tilt_series":22290,"frames_per_image":1036,"frame_range_min":null,"frame_range_max":null,"voxel_type":"BIT","pixel_width":0.573,"pixel_height":0.573,"micrographs_file_pattern":"data/spNOX_NADPHbound_aerobic/movies_*/*.eer","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Raw EER movies collected using a Krios G4 with a CFEG, Falcon 4 detector and Selectris X filter.\n\nThe EER movies contain 1036 frames and the total exposure dose of the sample was 70 e/A2. Magnification used was 215kx with a pixel size of 0.573 Å. Energy slit was set to 10 eV. \n\nXML files to generate optics groups are provided as well as the gain reference.","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[{"funding_body":"Max Planck Society","code":"N/A","country":"Germany"}],"version_history":[],"title":"Cryo-EM SPA dataset of Streptococcus pneumoniae NADPH oxidase with NADPH under turnover conditions","principal_investigator":[{"author_orcid":"0000-0001-6341-9368","middle_name":"J","organization":"Redox and Metalloproteins, Max Planck Insitute of Physics","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hesse","post_or_zip":"60438","telephone":null,"fax":null,"first_name":"Bonnie","last_name":"Murphy","email":"bonnie.murphy [at] biophys.mpg.de","country":"Germany","entry":"EMPIAR-11742"}],"status":"REL","deposition_date":"2023-10-16","release_date":"2025-07-10","obsolete_date":null,"update_date":"2025-07-10","corresponding_author":{"author":{"author_orcid":"0000-0001-6341-9368","middle_name":"J","organization":"Redox and Metalloproteins, Max Planck Insitute of Physics","street":"Max-von-Laue-Strasse 3","town_or_city":"Frankfurt am Main","state_or_province":"Hesse","post_or_zip":"60438","first_name":"Bonnie","last_name":"Murphy","country":"Germany"}},"authors":[{"author":{"name":"Dubach VRA","author_orcid":"0000-0002-1657-7184"}},{"author":{"name":"San Segundo-Acosta P","author_orcid":"0009-0000-1951-1423"}},{"author":{"name":"Murphy BJ","author_orcid":"0000-0001-6341-9368"}}],"cross_references":["EMD-18645"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Dubach VRA","author_orcid":"0000-0002-1657-7184"},{"name":"San Segundo-Acosta P","author_orcid":"0009-0000-1951-1423"},{"name":"Murphy BJ","author_orcid":"0000-0001-6341-9368"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase","volume":null,"country":"","first_page":null,"last_page":null,"year":"2024","language":"English","doi":"10.1038/s41594-024-01348-w","pubmedid":"39039317","details":"Raw micrograph frames recorded in EER format with a total dose of 70 e/Å using a Falcon IV detector in counting mode on a 300 kV Titan Krios G4 microscope. A total of 1036 EER frames were recorded and not gain corrected. Sample was frozen on a gold support grid (UltrAuFoil).","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":null,"preprint":false}],"dataset_size":"9.4 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8qt7"],"entry_doi":"10.6019/EMPIAR-11742"}}