{"EMPIAR-11687":{"imagesets":[{"segmentations":[],"name":"Raw multi-frame tilt images of Cytochalasin D and thapsigargin or MG132 treated protrusions of Drosophila S2 cells","directory":"data/Frames_Mdocs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":13,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.329,"pixel_height":1.329,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This folder contains raw, multi-frame tilt images. Data was collected on a K3 detector in super-resolution mode with a super-resolution pixel size of 1.329Å/pixel. Mdoc files and the gain reference are provided inside the folder.\nThis data is from the preliminary experiment shown in Fig. EV2A of the associated publication. TS_167, TS_169, TS_171, TS_172, TS_173 are from cells treated with 2.5uM Cytochalasin D (2h) and 25uM MG132 (5h). \nTS_178, TS_179, TS_180, TS_181, TS_182, TS_183, TS_185, TS_186 are from cells treated with 2.5uM Cytochalasin D (2h) and 2uM thapsigargin (5h).","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Binned, deconvolved tomograms of Cytochalasin D and thapsigargin or MG132 treated protrusions of Drosophila S2 cells","directory":"data/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":13,"frames_per_image":376,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":10.74,"pixel_height":10.74,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tomograms binned by 4 (from non-super resolution pixel size), reconstructed and deconvolved in WARP. \nThis data is from the preliminary experiment shown in Fig. EV2A of the associated publication. TS_167, TS_169, TS_171, TS_172, TS_173 are from cells treated with 2.5uM Cytochalasin D (2h) and 25uM MG132 (5h). \nTS_178, TS_179, TS_180, TS_181, TS_182, TS_183, TS_185, TS_186 are from cells treated with 2.5uM Cytochalasin D (2h) and 2uM thapsigargin (5h).","image_width":"1022","image_height":"1440"}],"workflow_file":null,"grant_references":[{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MRC_UP_A025_1011","country":"United Kingdom"},{"funding_body":"Wellcome Trust","code":"210711/Z/18/Z","country":"United Kingdom"}],"version_history":[],"title":"Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells treated with thapsigargin or MG132","principal_investigator":[{"author_orcid":"0000-0001-7292-5430","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":null,"town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Andrew","last_name":"Carter","email":"cartera [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-11687"}],"status":"REL","deposition_date":"2023-09-18","release_date":"2023-10-03","obsolete_date":null,"update_date":"2023-10-03","corresponding_author":{"author":{"author_orcid":"0000-0002-4406-5151","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":null,"town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"CB2 0QH","first_name":"Camilla","last_name":"Ventura Santos","country":"United Kingdom"}},"authors":[{"author":{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"}},{"author":{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"}}],"cross_references":["EMD-18475"],"biostudies_references":[],"idr_references":[],"empiar_references":[{"name":"EMPIAR-11450"},{"name":"EMPIAR-11451"},{"name":"EMPIAR-11452"},{"name":"EMPIAR-11453"}],"citation":[{"authors":[{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"},{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"},{"name":"Rogers SL","author_orcid":"0000-0002-3941-9912"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"CryoET shows cofilactin filaments inside the microtubule lumen","volume":null,"country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.15252/embr.202357264","pubmedid":"37702953","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"EMBO Reports","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"107.6 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11687"}}