{"EMPIAR-11662":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM.","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2540,"frames_per_image":25,"frame_range_min":1,"frame_range_max":25,"voxel_type":"32 BIT FLOAT","pixel_width":1.055,"pixel_height":1.055,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 10 s exposures were captured in 25 frames with a total dose of ~44 e/Å-2. A pixel size of 1.055 Å/pixel was used.","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_U105663141","country":"United Kingdom"},{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_UU_00015/2","country":"United Kingdom"}],"version_history":[],"title":"Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I","principal_investigator":[{"author_orcid":"0000-0001-8667-6797","middle_name":null,"organization":"MRC Mitochondrial Biology Unit","street":"Hills Road","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0XY","telephone":null,"fax":null,"first_name":"Judy","last_name":"Hirst","email":"jh [at] mrc-mbu.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-11662"}],"status":"REL","deposition_date":"2023-08-02","release_date":"2024-06-27","obsolete_date":null,"update_date":"2024-06-27","corresponding_author":{"author":{"author_orcid":"0000-0001-8667-6797","middle_name":null,"organization":"MRC Mitochondrial Biology Unit","street":"Hills Road","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0XY","first_name":"Judy","last_name":"Hirst","country":"United Kingdom"}},"authors":[{"author":{"name":"Grba DN","author_orcid":"0000-0003-2915-951X"}},{"author":{"name":"Hirst J","author_orcid":"0000-0001-8667-6797"}}],"cross_references":["EMD-10815","EMD-10816"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Grba DN","author_orcid":"0000-0003-2915-951X"},{"name":"Hirst J","author_orcid":"0000-0001-8667-6797"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Mitochondrial complex I structure reveals ordered water molecules for catalysis and proton translocation","volume":"27","country":"","first_page":"892","last_page":"900","year":"2020","language":"English","doi":"10.1038/s41594-020-0473-x","pubmedid":"32747785","details":"Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 10 s exposures were captured in 25 frames with a total dose of ~44 e/Å-2. A pixel size of 1.055 Å/pixel was used.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":"10","preprint":false}],"dataset_size":"3.3 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["6yj4","6yj5"],"entry_doi":"10.6019/EMPIAR-11662"}}