{"EMPIAR-11604":{"imagesets":[{"segmentations":[],"name":"Motion corrected, dose-weighted micrographs from two data collection sessions","directory":"data","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3046,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.89,"pixel_height":0.89,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"This is a dataset of the AP2 clathrin adaptor in the presence of heparin. Included are aligned and dose weighted micrographs from cryoSPARC v3.\n\nTwo data collections were performed one week apart with identical microscope parameters. The data are in two separate directories split by collection date.\n\nTotal micrographs: 3046\nPixel Size: 0.89 Ang\nMagnification = 45,000","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R01GM127548","country":"United States"}],"version_history":[],"title":"AP2 in the presence of heparin","principal_investigator":[{"author_orcid":"0000-0003-1136-6000","middle_name":"Wayne","organization":"University of North Carolina at Chapel Hill","street":"3049A Genetic Medicine Bldg","town_or_city":"Chapel Hill","state_or_province":"NC","post_or_zip":"27599","telephone":"9198439585","fax":null,"first_name":"Richard","last_name":"Baker","email":"baker [at] med.unc.edu","country":"United States","entry":"EMPIAR-11604"}],"status":"REL","deposition_date":"2023-07-05","release_date":"2023-08-18","obsolete_date":null,"update_date":"2023-08-18","corresponding_author":{"author":{"author_orcid":"0000-0003-1136-6000","middle_name":"Wayne","organization":"University of North Carolina at Chapel Hill","street":"3049A Genetic Medicine Bldg","town_or_city":"Chapel Hill","state_or_province":"NC","post_or_zip":"27599","first_name":"Richard","last_name":"Baker","country":"United States"}},"authors":[{"author":{"name":"Partlow EA","author_orcid":"0000-0001-5513-088X"}},{"author":{"name":"Cannon KS","author_orcid":"0000-0002-6648-3442"}},{"author":{"name":"Hollopeter G","author_orcid":"0000-0002-6409-0530"}},{"author":{"name":"Baker RW","author_orcid":"0000-0003-1136-6000"}}],"cross_references":["EMD-24711","EMD-24710"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Partlow EA","author_orcid":"0000-0001-5513-088X"},{"name":"Cannon KS","author_orcid":"0000-0002-6648-3442"},{"name":"Hollopeter G","author_orcid":"0000-0002-6409-0530"},{"name":"Baker RW","author_orcid":"0000-0003-1136-6000"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis of an endocytic checkpoint that primes the AP2 clathrin adaptor for cargo internalization","volume":"29","country":"","first_page":"339","last_page":"347","year":"2022","language":"English","doi":"10.1038/s41594-022-00749-z","pubmedid":"35347313","details":"The micrographs are motion corrected and dose-weighted in cryoSPARC v3. The input movies were dose-fractionated stacks collected on a Talos Arctica with a K3 DED. The movies originally contained 60 frames, with a pixel size of 0.89 Angstrom, and a total dose of 55 e-/A^2. These micrographs were used to determine a structure of AP2 in the presence of heparin.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":"4","preprint":false}],"dataset_size":"267.5 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7rw9","7rw8"],"entry_doi":"10.6019/EMPIAR-11604"}}