{"EMPIAR-11560":{"imagesets":[{"segmentations":[],"name":"Unaligned raw frames of mini shell co-expressing CsoS1A, CsoS4A and truncated CsoS2 (C1 construct)","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":13151,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.831,"pixel_height":0.831,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The micrographs are raw data frames with 40 fractions, collected with Gatan K3 camera in super-resolution but 2x binned in EPU. The gain reference is in the same dimension as the 2x binned micrographs.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Wellcome Trust","code":"206422/Z/17/Z","country":"United Kingdom"},{"funding_body":"Biotechnology and Biological Sciences Research Council (BBSRC)","code":"BBSRC: BB/S003339/1, BB/V009729/1 and BB/R003890/1","country":"United Kingdom"},{"funding_body":"National Natural Science Foundation of China (NSFC)","code":"National Natural Science Foundation of China (32070109)","country":"China"},{"funding_body":"European Research Council (ERC)","code":"ERC AdG grant (101021133)","country":"United Kingdom"},{"funding_body":"Royal Society","code":"Royal Society (URF\\R\\180030, RGF\\EA\\181061 and RGF\\EA\\180233),","country":"United Kingdom"}],"version_history":[],"title":"Carboxysome minishell containing CsoS1A4A and truncated CsoS2","principal_investigator":[{"author_orcid":"0000-0003-1803-691X","middle_name":null,"organization":"The University of Oxford","street":null,"town_or_city":"Oxford","state_or_province":null,"post_or_zip":"OX3 7BN","telephone":null,"fax":null,"first_name":"Peijun","last_name":"Zhang","email":"peijun.zhang [at] strubi.ox.ac.uk","country":"United Kingdom","entry":"EMPIAR-11560"}],"status":"REL","deposition_date":"2023-05-03","release_date":"2023-09-22","obsolete_date":null,"update_date":"2023-09-22","corresponding_author":{"author":{"author_orcid":"0000-0001-7268-0306","middle_name":null,"organization":"The University of Hong Kong","street":null,"town_or_city":"Hong Kong","state_or_province":null,"post_or_zip":"000000","first_name":"Tao","last_name":"Ni","country":"Hong Kong"}},"authors":[{"author":{"name":"Ni T","author_orcid":"0000-0001-7268-0306"}},{"author":{"name":"Jiang Q","author_orcid":null}},{"author":{"name":"Ng PC","author_orcid":"0000-0003-0905-0584"}},{"author":{"name":"Dou H","author_orcid":null}},{"author":{"name":"Liu LN","author_orcid":"0000-0002-8884-4819"}},{"author":{"name":"Zhang P","author_orcid":"0000-0003-1803-691X"}}],"cross_references":["EMD-15595","EMD-15719","EMD-15724","EMD-15720","EMD-15723","EMD-15722"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Ni T","author_orcid":"0000-0001-7268-0306"},{"name":"Jiang Q","author_orcid":null},{"name":"Ng PC","author_orcid":"0000-0003-0905-0584"},{"name":"Shen J","author_orcid":null},{"name":"Dou H","author_orcid":null},{"name":"Zhu Y","author_orcid":"0000-0003-1025-7929"},{"name":"Radecke J","author_orcid":"0000-0002-5815-5537"},{"name":"Dykes GF","author_orcid":"0000-0002-0626-9487"},{"name":"Huang F","author_orcid":null},{"name":"Liu LN","author_orcid":"0000-0002-8884-4819"},{"name":"Zhang P","author_orcid":"0000-0003-1803-691X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly","volume":"14","country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1038/s41467-023-41211-y","pubmedid":"37679318","details":"The mini-shell-4 samples which is obtained by co-expression of CsoS1A, CsoS4A and CosS2 C1 mutant are imaged.The cryoEM sample grids were prepared using Vitrobot. Three microliters of mini-shell samples were applied to glow-discharged Quantifoil grids (R2/1) and blotted with filter paper for 3 seconds before plunge freezing with liquid nitrogen-cooled ethane. The temperature was set to 20 ℃ and humidity at 100% during plunge freezing. The micrographs were taken using Krios microscope equipped with a Gatan K3 director electron camera and BioQuantum energy filter (slit width 20 eV). Total exposure dose is 44 electrons/angstrom2, fractionated into 50 frames in tif format. The raw movies are not gain normalised.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"2.2 TB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-11560"}}