{"EMPIAR-11537":{"imagesets":[{"segmentations":[],"name":"Raw FIB SEM Run 1","directory":"data/4G_Run 1","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3419,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":50.0,"pixel_height":50.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"First part of the raw data (out of 3 in total).\nImages were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.","image_width":"14976","image_height":"14976"},{"segmentations":[],"name":"Run 2","directory":"data/4G_Run 2","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":156,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":50.0,"pixel_height":50.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Second part of the raw data (out of 3 in total).\nImages were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.","image_width":"14976","image_height":"14976"},{"segmentations":[],"name":"Run 3","directory":"data/Run 3","category":"micrographs - single frame","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4266,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":50.0,"pixel_height":50.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Third part of the raw data (out of 3 in total).\nImages were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.","image_width":"14976","image_height":"14976"},{"segmentations":[],"name":"Downscaled (20nm) aligned FIB SEM stack resliced to match the Airyscan dataset","directory":"data/Downscaled20nm","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1,"frames_per_image":258,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":200.0,"pixel_height":200.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Processed and aligned stack of FIB SEM images downscaled to 20 nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies","image_width":"3213","image_height":"1959"},{"segmentations":[],"name":"Aligned full resolution FIB SEM stack resliced to match the Airyscan dataset","directory":"data/5nm","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":1,"frames_per_image":900,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":50.0,"pixel_height":50.0,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Processed and aligned stack of FIB SEM images at full 5nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies","image_width":"12850","image_height":"7835"}],"workflow_file":null,"grant_references":[{"funding_body":"The Francis Crick Institute","code":"FC001999","country":"United Kingdom"}],"version_history":[],"title":"Benchmark FIB SEM data (#2) of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy","principal_investigator":[{"author_orcid":"0000-0002-5703-2922","middle_name":null,"organization":"Francis Crick Institute, Electron Microscopy STP","street":"1 Midland Road","town_or_city":"London","state_or_province":"London","post_or_zip":"NW1 1AT","telephone":null,"fax":null,"first_name":"Marie-Charlotte","last_name":"Domart","email":"marie-charlotte.domart [at] crick.ac.uk","country":"United Kingdom","entry":"EMPIAR-11537"}],"status":"REL","deposition_date":"2023-04-27","release_date":"2023-05-22","obsolete_date":null,"update_date":"2023-05-23","corresponding_author":{"author":{"author_orcid":"0000-0002-5703-2922","middle_name":null,"organization":"Francis Crick Institute, Electron Microscopy STP","street":"1 Midland Road","town_or_city":"London","state_or_province":"London","post_or_zip":"NW1 1AT","first_name":"Marie-Charlotte","last_name":"Domart","country":"United Kingdom"}},"authors":[{"author":{"name":"Peddie CJ","author_orcid":"0000-0002-8329-5419"}},{"author":{"name":"Domart MC","author_orcid":"0000-0002-5703-2922"}},{"author":{"name":"Collinson LM","author_orcid":null}}],"cross_references":[],"biostudies_references":[{"name":"S-BSST1075"}],"idr_references":[],"empiar_references":[{"name":"EMPIAR-10819"}],"citation":[{"authors":[{"name":"Krentzel D","author_orcid":"0000-0002-6234-7259"},{"name":"Elphick M","author_orcid":"0000-0003-4453-5691"},{"name":"Domart M","author_orcid":"0000-0002-5703-2922"},{"name":"Peddie CJ","author_orcid":"0000-0002-8329-5419"},{"name":"Laine RF","author_orcid":null},{"name":"Henriques R","author_orcid":null},{"name":"Collinson LM","author_orcid":null},{"name":"Jones ML","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"CLEM-Reg: An automated point cloud based registration algorithm for correlative light and volume electron microscopy","volume":null,"country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1101/2023.05.11.540445","pubmedid":null,"details":"Raw and processed benchmark FIB SEM data matching Airyscan data deposited on Biostudies","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"511.9 GB","experiment_type":"CLEM","scale":"cell","entry_doi":"10.6019/EMPIAR-11537"}}