{"EMPIAR-11521":{"imagesets":[{"segmentations":[],"name":"Unaligned micrographs (raw movies)","directory":"data/micrographs_multiframe","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7101,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"data/micrographs_multiframe/Es54-dhsU36mm2_*_000?.tif","picked_particles_file_pattern":"data/aux_files/EsN-dhsU36mm2_particles_picked.star","picked_particles_directory":"","details":"FEI Titan Krios + Gatan K3, super-resolution, image shift, BioQuantum energy filter 20 eV slit, Cs correction; accelerating voltage 300 kV; dose 30 e-/px(detector)/s; dose 7.5 e-/px(image)/s; pixel size 1.08 A/px(detector); pixel size 0.54 A/px(image); exposure 2.5 s; dose/frame 1.286008 e-/A^2/frame; frame time 0.05 s; total dose 64.30041 e-/A^2; total frames 50; defocus range -1 to -2.5um; gain correction was applied on the fly during collection\nPicked particles star file \"EsN-dhsU36mm2_particles_picked.star\" (\"aux_files\" folder) contains all particles picked initially and is only provided for reference.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Motion-corrected micrographs","directory":"data/micrographs_singleframe","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7101,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/micrographs_singleframe/Es54-dhsU36mm2_*_000?.mrc","picked_particles_file_pattern":"data/aux_files/EsN-dhsU36mm2_particles_picked.star","picked_particles_directory":"","details":"See also the \"corrected_micrographs.star\" file in the \"aux_files\" folder.\nRaw micrographs were binned by 2 during motion correction.\nPicked particles star file \"EsN-dhsU36mm2_particles_picked.star\" (\"aux_files\" folder) contains all particles picked initially and is only provided for reference.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Non-dose-weighted motion-corrected micrographs","directory":"data/micrographs_singleframe_noDW","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7101,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/micrographs_singleframe_noDW/Es54-dhsU36mm2_*_000?_noDW.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"non-dose-weighted micrographs from motioncor2 output","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Particle images for map 1","directory":"data/particles_shiny_map1","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":7018,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/particles_shiny_map1/Es54-dhsU36mm2_*_000?_shiny.mrcs","picked_particles_file_pattern":"data/aux_files/EsN-dhsU36mm2_particles_map1.star","picked_particles_directory":"data/particles_shiny_map1","details":"Particle stack for map 1 after Bayesian polishing (see \"EsN-dhsU36mm2_particles_map1.star\" (final refinement parameters) and \"EsN-dhsU36mm2_particles_shiny.star\" (output from Bayesian Polishing) in \"aux_files\" folder)","image_width":"256","image_height":"256"},{"segmentations":[],"name":"Particle images for map 2 (subtracted particles)","directory":"data/particles_subtracted_map2","category":"picked particles - single frame - processed","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":398,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"data/particles_subtracted_map2/subtracted_particles_batch_*.mrcs","picked_particles_file_pattern":"data/aux_files/EsN-dhsU36mm2_particles_map2.star","picked_particles_directory":"data/particles_subtracted_map2","details":"Particle stack for map 2 after masked subtraction (see \"EsN-dhsU36mm2_particles_map2.star\" (final refinement parameters) and \"EsN-dhsU36mm2_particles_original_map2.star\" (original particles) in \"aux_files\" folder)","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health (NIH)","code":"R35 GM118130","country":"United States"}],"version_history":[],"title":"CryoEM of E. coli RNA polymerase Sigma N bound to mismatch DNA fragment dhsU36mm2 (-12A)","principal_investigator":[{"author_orcid":"0000-0002-8241-3153","middle_name":null,"organization":"The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Seth","last_name":"Darst","email":"darst [at] rockefeller.edu","country":"United States","entry":"EMPIAR-11521"}],"status":"REL","deposition_date":"2023-04-05","release_date":"2023-05-16","obsolete_date":null,"update_date":"2023-05-16","corresponding_author":{"author":{"author_orcid":"0000-0003-3002-3695","middle_name":null,"organization":"The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"New York","post_or_zip":"10065","first_name":"Andreas","last_name":"Mueller","country":"United States"}},"authors":[{"author":{"name":"Mueller AU","author_orcid":"0000-0003-3002-3695"}},{"author":{"name":"Chen J","author_orcid":"0000-0002-2311-003X"}},{"author":{"name":"Wu M","author_orcid":"0000-0002-2248-9383"}},{"author":{"name":"Chiu C","author_orcid":null}},{"author":{"name":"Nixon BT","author_orcid":"0000-0002-3568-5703"}},{"author":{"name":"Campbell EA","author_orcid":"0000-0002-1332-128X"}},{"author":{"name":"Darst SA","author_orcid":"0000-0002-8241-3153"}}],"cross_references":["EMD-28783","EMD-28784","EMD-28785"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Mueller AU","author_orcid":"0000-0003-3002-3695"},{"name":"Chen J","author_orcid":"0000-0002-2311-003X"},{"name":"Wu M","author_orcid":"0000-0002-2248-9383"},{"name":"Chiu C","author_orcid":null},{"name":"Nixon BT","author_orcid":"0000-0002-3568-5703"},{"name":"Campbell EA","author_orcid":"0000-0002-1332-128X"},{"name":"Darst SA","author_orcid":"0000-0002-8241-3153"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"A general mechanism for transcription bubble nucleation in bacteria","volume":"120","country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1073/pnas.2220874120","pubmedid":"36972428","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Proceedings of the National Academy of Sciences of the United States of America","journal_abbreviation":"Proc Natl Acad Sci U S A","issue":"14","preprint":false}],"dataset_size":"8.2 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8f1j"],"entry_doi":"10.6019/EMPIAR-11521"}}