{"EMPIAR-11520":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of tilt series of chloramphenicol treated Mycoplasma pneumoniae cells","directory":"data/Cm_K3_data/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":139,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.329,"pixel_height":1.329,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"5808 unaligned multiframe micrographs in LZW compressed TIFF format of 139 tilt series of chloramphenicol treated M. pneumoniae cells. Data collected with a Gatan K3 camera in non-CDS mode, magnification 64k, and 3.34 e-/pixel dose per tilt. Gain reference needs flipping along the Y axis. SerialEM mdoc files and the IMOD/etomo alignment files (preferentially with motion correction and raw data processing performed in Warp) are provided.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Chan Zuckerberg Initiative","code":"Visual Proteomics","country":"Germany"}],"version_history":[],"title":"Tilt series of Mycoplasma pneumoniae cells treated with chloramphenicol collected on Gatan K3 camera","principal_investigator":[{"author_orcid":"0000-0001-6968-041X","middle_name":null,"organization":"European Molecular Biology Laboratory(EMBL)","street":"Meyerhofstrasse 1","town_or_city":"Heidelberg","state_or_province":"Germany (DEU)","post_or_zip":"69126","telephone":"+49 (0)62213878451","fax":null,"first_name":"Julia","last_name":"Mahamid","email":"julia.mahamid [at] embl.de","country":"Germany","entry":"EMPIAR-11520"}],"status":"REL","deposition_date":"2023-04-18","release_date":"2024-11-06","obsolete_date":null,"update_date":"2024-11-06","corresponding_author":{"author":{"author_orcid":"0000-0003-4368-2526","middle_name":null,"organization":"European Molecular Biology Laboratory(EMBL)","street":"Meyerhofstrasse 1","town_or_city":"Heidelberg","state_or_province":"Germany (DEU)","post_or_zip":"69126","first_name":"Liang","last_name":"Xue","country":"Germany"}},"authors":[{"author":{"name":"Xue L","author_orcid":"0000-0003-4368-2526"}},{"author":{"name":"Spahn CMT","author_orcid":"0000-0002-2939-9487"}},{"author":{"name":"Schacherl M","author_orcid":"0000-0002-5478-2509"}},{"author":{"name":"Mahamid J","author_orcid":"0000-0001-6968-041X"}}],"cross_references":["EMD-17132"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Xue L","author_orcid":"0000-0003-4368-2526"},{"name":"Spahn CMT","author_orcid":"0000-0002-2939-9487"},{"name":"Schacherl M","author_orcid":"0000-0002-5478-2509"},{"name":"Mahamid J","author_orcid":"0000-0001-6968-041X"}],"editors":[{"name":"Mahamid J","author_orcid":"0000-0001-6968-041X"}],"published":false,"j_or_nj_citation":true,"title":"Structural evidence of context-dependent action of chloramphenicol in cells","volume":null,"country":"Germany","first_page":null,"last_page":null,"year":"2023","language":"English","doi":null,"pubmedid":null,"details":"Dose-symmetric tilt series of intact M. pneumoniae cells treated with chloramphenicol. Gold fiducials were added before plunge freezing. Data were collected on a Titan Krios microscope equipped with Gatan K3 camera (Magnification 64,000x, pixel size 1.329 Å,  tilt range -60°:3°:60°, camera in non-CDS counting mode, 10 frames per tilt image, constant exposure time for each tilt and total dose 137 e/Å2, defocus range from -1 to -3.25μm). Raw frames (movie) for every tilt images of the 139 tilt series were deposited. Gain reference needs flipping along the Y axis in Warp. Detailed information can be found in the provided mdoc files. IMOD/etomo alignment files are also provided (preferentially with motion correction and raw data processing performed in Warp).","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"170.5 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11520"}}