{"EMPIAR-11513":{"imagesets":[{"segmentations":[],"name":"K3 Movies (.tif) for 2-36 Antibody + SARS-CoV-2 Spike","directory":"data/SARS-CoV-2-Spike-dataset-movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4135,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.07,"pixel_height":1.07,"micrographs_file_pattern":"data/SARS-CoV-2-Spike-dataset-movies/20aug25a/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"K3 Movies (.tif) for 2-36 Antibody + SARS-CoV Spike","directory":"data/SARS-CoV-Spike-dataset-movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4980,"frames_per_image":60,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.07,"pixel_height":1.07,"micrographs_file_pattern":"data/SARS-CoV-Spike-dataset-movies/20oct30a/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM SPA datasets of broadly neutralizing antibody 2-36 in complex with prefusion SARS-CoV-2 and SARS-CoV spike glycoproteins (K3 movies/.tif files)","principal_investigator":[{"author_orcid":"0000-0001-9943-8819","middle_name":null,"organization":"Department of Biochemistry, Columbia University","street":"3227 Broadway","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","telephone":null,"fax":null,"first_name":"Lawrence","last_name":"Shapiro","email":"lss8 [at] columbia.edu","country":"United States","entry":"EMPIAR-11513"}],"status":"REL","deposition_date":"2022-11-16","release_date":"2023-05-09","obsolete_date":null,"update_date":"2023-05-11","corresponding_author":{"author":{"author_orcid":"0000-0002-2910-0908","middle_name":"Gavin","organization":"Columbia University","street":"3227 Broadway","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","first_name":"Ryan","last_name":"Casner","country":"United States"}},"authors":[{"author":{"name":"Casner RG","author_orcid":"0000-0002-2910-0908"}},{"author":{"name":"Cerutti G","author_orcid":"0000-0002-9081-7561"}},{"author":{"name":"Shapiro L","author_orcid":"0000-0001-9943-8819"}}],"cross_references":["EMD-24190"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Wang P","author_orcid":"0000-0003-2454-7652"},{"name":"Casner RG","author_orcid":"0000-0002-2910-0908"},{"name":"Nair MS","author_orcid":"0000-0002-5994-3957"},{"name":"Yu J","author_orcid":null},{"name":"Guo Y","author_orcid":null},{"name":"Wang M","author_orcid":null},{"name":"Chan JF","author_orcid":"0000-0001-6336-6657"},{"name":"Cerutti G","author_orcid":"0000-0002-9081-7561"},{"name":"Iketani S","author_orcid":"0000-0002-3733-9556"},{"name":"Liu L","author_orcid":null},{"name":"Sheng Z","author_orcid":null},{"name":"Chen Z","author_orcid":"0000-0002-4511-2888"},{"name":"Yuen KY","author_orcid":"0000-0002-2083-1552"},{"name":"Kwong PD","author_orcid":null},{"name":"Huang Y","author_orcid":null},{"name":"Shapiro L","author_orcid":"0000-0001-9943-8819"},{"name":"Ho DD","author_orcid":"0000-0003-1627-149X"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses","volume":"11","country":"","first_page":"147","last_page":"157","year":"2022","language":"English","doi":"10.1080/22221751.2021.2011623","pubmedid":"34836485","details":"Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Emerging microbes & infections","journal_abbreviation":"Emerg Microbes Infect","issue":"1","preprint":false}],"dataset_size":"2.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7n5h"],"entry_doi":"10.6019/EMPIAR-11513"}}