{"EMPIAR-11472":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 1","directory":"data/Tifs_DS1_MK67I-GFP","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":44352,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 2","directory":"data/Tifs_DS2_MK67I-GFP","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":45846,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 3","directory":"data/Tifs_DS3_MK67I-GFP","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":44335,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 4","directory":"data/Tifs_DS4_MK67I-GFP","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":37166,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Polished particle stack of human nucleolar and nuclear pre-60S assembly intermediates","directory":"data/Particle_stack","category":"micrographs - single frame","header_format":"MRCS","data_format":"MRCS","num_images_or_tilt_series":2474967,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.08,"pixel_height":1.08,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Merged set of pre-60S particles from four datasets. Particles are polished, magnification anisotropy corrected and per-particle defocus corrected. A Relion .star format file is provided and contains the final Euler angles, shifts and also the micrograph coordinates.","image_width":"480","image_height":"480"}],"workflow_file":null,"grant_references":[{"funding_body":"European Molecular Biology Organization (EMBO)","code":"ALTF 711-2019","country":"Germany"},{"funding_body":"The G. Harold and Leila Y. Mathers Foundation","code":"MF-2104-01554","country":"United States"}],"version_history":[],"title":"Single particle cryo-EM dataset of human nucleolar and nuclear pre-60S assembly intermediates","principal_investigator":[{"author_orcid":"0000-0002-9373-4737","middle_name":null,"organization":"Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Sebastian","last_name":"Klinge","email":"klinge [at] rockefeller.edu","country":"United States","entry":"EMPIAR-11472"}],"status":"REL","deposition_date":"2022-12-06","release_date":"2023-09-26","obsolete_date":null,"update_date":"2023-09-26","corresponding_author":{"author":{"author_orcid":"0000-0003-0930-4249","middle_name":null,"organization":"Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University","street":"1230 York Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10065","first_name":"Arnaud","last_name":"Vanden Broeck","country":"United States"}},"authors":[{"author":{"name":"Vanden Broeck A","author_orcid":"0000-0003-0930-4249"}},{"author":{"name":"Klinge S","author_orcid":"0000-0002-9373-4737"}}],"cross_references":["EMD-29153","EMD-29199","EMD-29198","EMD-29206","EMD-29150","EMD-29177","EMD-29167","EMD-29277","EMD-29149","EMD-29202"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Vanden Broeck A","author_orcid":"0000-0003-0930-4249"},{"name":"Klinge S","author_orcid":"0000-0002-9373-4737"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Principles of human pre-60<i>S</i> biogenesis","volume":"381","country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1126/science.adh3892","pubmedid":"37410842","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":"6653","preprint":false}],"dataset_size":"140.5 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8flf"],"entry_doi":"10.6019/EMPIAR-11472"}}