{"EMPIAR-11466":{"imagesets":[{"segmentations":[],"name":"Unaligned movies of the Cas7-11-crRNA-Csx29 complex (Dataset_inactive_CASP)","directory":"data/EMPIAR_CASP/Dataset_inactive_CASP","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":16553,"frames_per_image":31,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.6632,"pixel_height":0.6632,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned, non-gain-normalised, 31-frame movies from a Bioquantum-K3 camera recorded in super-resolution mode with twofold binning at 130,000× magnification. 16553 movies were collected with EPU on the MIT.nano Titan Krios G3i operated at 300 kV. Each micrograph accumulates 40 electrons per square Angstrom over 31 frames (0.69 s). Nominal pixel size is 0.6632 Å/pixel. Gain reference image for the dataset is attached. Final refinement star files produced from focused refinements for each reconstruction deposited on EMDB are also attached: EMD-28064 at 3.0 Å resolution, and EMD-28070 at 3.2 Å resolution. Data was processed in RELION 4.0.\n\nFurther details about the dataset:\n\nGrid: Quantifoil R1.2/1.3 on 300 copper mesh\nC2 aperture: 50 um\nSpot: 4\nBeam diameter: 0.75 um\nEF slit: 20 eV\nAcquisition pattern: 4 shots/hole x 12 um AFIS\nPhysical Pixel: 0.6632 A/pix\nSuperRes pixel: 0.3316 A/pix\nExposure rate: 25.53 e/pix/s\nExposure time: 0.69 s\nTotal exposure: 40.07 e/A2 \nFrames: 31\nDefocus: 0.5 - 2 um","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned movies of the Cas7-11-crRNA-target RNA-Csx29-Csx30 complex (Dataset_active_CASP)","directory":"data/EMPIAR_CASP/Dataset_active_CASP","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":32890,"frames_per_image":31,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.6632,"pixel_height":0.6632,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned, non-gain-normalised, 31-frame movies from a Bioquantum-K3 camera recorded in super-resolution mode with twofold binning at 130,000× magnification. 32890 movies were collected with EPU on the MIT.nano Titan Krios G3i operated at 300 kV. Each micrograph accumulates 40 electrons per square Angstrom over 31 frames (0.69 s). Nominal pixel size is 0.6632 Å/pixel. Gain reference image for the dataset is attached. Final refinement star files produced from focused refinements for each reconstruction deposited on EMDB are also attached: EMD-28065 at 2.5 Å resolution, EMD-28071 at 2.8 Å resolution, EMD-28072 at 2.6 Å resolution, and EMD-28073 at 2.7 Å resolution. Only one-third of the dataset was used during processing in RELION 4.0.\n\nFurther details about the dataset:\n\nGrid: Quantifoil R1.2/1.3 on 300 copper mesh\nC2 aperture: 50 um\nSpot: 4\nBeam diameter: 0.75 um\nEF slit: 20 eV\nAcquisition pattern: 4 shots/hole x 12 um AFIS\nPhysical Pixel: 0.6632 A/pix\nSuperRes pixel: 0.3316 A/pix\nExposure rate: 25.53 e/pix/s\nExposure time: 0.69 s\nTotal exposure: 40.07 e/A2 \nFrames: 31\nDefocus: 0.5 - 2 um","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"National Institutes of Health/National Eye Institute (NIH/NEI)","code":"1DP1-HL141201","country":"United States"},{"funding_body":"National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)","code":"2R01HG009761-05","country":"United States"}],"version_history":[],"title":"Desulfonema ishimotonii Cas7-11 in complex with Csx29 and Csx30","principal_investigator":[{"author_orcid":"0000-0002-3866-2742","middle_name":null,"organization":"Broad Institute","street":null,"town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"02142","telephone":null,"fax":null,"first_name":"Fatma Esra","last_name":"Demircioglu","email":"fdemirci [at] broadinstitute.org","country":"United States","entry":"EMPIAR-11466"}],"status":"REL","deposition_date":"2023-03-09","release_date":"2023-04-11","obsolete_date":null,"update_date":"2023-04-11","corresponding_author":{"author":{"author_orcid":"0000-0002-3866-2742","middle_name":null,"organization":"Broad Institute","street":null,"town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"02142","first_name":"Fatma Esra","last_name":"Demircioglu","country":"United States"}},"authors":[{"author":{"name":"Strecker J","author_orcid":"0000-0001-8953-5615"}},{"author":{"name":"Demircioglu FE","author_orcid":"0000-0002-3866-2742"}},{"author":{"name":"Li D","author_orcid":"0000-0002-8921-5168"}},{"author":{"name":"Faure G","author_orcid":"0000-0001-9537-2277"}},{"author":{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"}},{"author":{"name":"Macrae RK","author_orcid":"0000-0002-7850-9056"}},{"author":{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}}],"cross_references":["EMD-28065","EMD-28064","EMD-28071","EMD-28072","EMD-28073","EMD-28070"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Strecker J","author_orcid":"0000-0001-8953-5615"},{"name":"Demircioglu FE","author_orcid":"0000-0002-3866-2742"},{"name":"Li D","author_orcid":"0000-0002-8921-5168"},{"name":"Faure G","author_orcid":"0000-0001-9537-2277"},{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"},{"name":"Gootenberg JS","author_orcid":"0000-0003-2757-2175"},{"name":"Abudayyeh OO","author_orcid":"0000-0002-7979-3220"},{"name":"Nishimasu H","author_orcid":"0000-0002-2505-1416"},{"name":"Macrae RK","author_orcid":"0000-0002-7850-9056"},{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"RNA-activated protein cleavage with a CRISPR-associated endopeptidase","volume":"378","country":"United States","first_page":"874","last_page":"881","year":"2022","language":"English","doi":"10.1126/science.add7450","pubmedid":"36423276","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science","journal_abbreviation":"","issue":"6622","preprint":false}],"dataset_size":"5.7 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8eey","8eex"],"entry_doi":"10.6019/EMPIAR-11466"}}