{"EMPIAR-11458":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame movies of Bombyx mori R2 retrotransposon initiating target-primed reverse transcription","directory":"data/Movies/Images-Disc1","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":16551,"frames_per_image":40,"frame_range_min":1,"frame_range_max":40,"voxel_type":"UNSIGNED BYTE","pixel_width":0.663,"pixel_height":0.663,"micrographs_file_pattern":"data/Movies/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff","picked_particles_file_pattern":"data/Movies/refined_particles_data.star","picked_particles_directory":"","details":"This entry contains 16551 K3 movies in compressed tiff format\n\nCollection info:\nTitan Krios G3i\nVoltage: 300 keV\nCs: 2.7 mm\n\nGatan K3 detector\n130000x magnification\n0.663 A/pix\n20 eV energy filter\n100 uM objective aperture\n\n42.2 e/A2 over 40 frames\n\nGain reference: K3-GainRef-bin2.mrc\nFor RELION, use gain rotation 0 (no rotation) and gain flip 1 (flip upside down)\n\n\nrefined_particles_data.star is the run_data.star file for the final refinement\n\nNote: many of the particles did not align well, so the final refinement used a very small fraction of the full data set. You might have better luck though!\n\nSample: Bombyx mori R2 retrotransposon initiating target-primed reverse transcription\nGrid: Quantifoil Cu 300 mesh R2/2 + 2 nm carbon\nCemera: Gatan K3 post a BioQuantum energy filter\nCamera mode: super resolution, 2-fold binning by EPU\nTEM mode: EFTEM NanoProbe\nMag: x130k\nC2 aperture: 50 um\nSpot: 4\nBeam diameter: 0.75 um\nEF slit: 20 eV\nData collection software: EPU\nAcquisition method: AFIS\nAcquisition pattern: 9 images per hole, 12 um distance per stage shift\nPhysical Pixel: 0.663 A/pix\nSuperRes pixel: 0.3315 A/pix\nExposure rate: 26.9 e/pix/s\nExposure time: 0.69 s\nTotal exposure: 42.2 e/A2\nFrames: 40\nDefocus: 1.5 - 2.5 um\nMovies: 16551\nData format: non-gain-normalized LZW compressed TIFFs\nGain reference: \"K3-GainRef-bin2.mrc\"\nFinal particles: 39616\nParticles file: \"refined_particles_data.star\"","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Howard Hughes Medical Institute (HHMI)","code":"NA","country":"United States"},{"funding_body":"National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)","code":"R01HG009761","country":"United States"}],"version_history":[],"title":"Cryo-EM micrographs for the Bombyx mori R2 retrotransposon initiating target-primed reverse transcription","principal_investigator":[{"author_orcid":"0000-0003-0178-7995","middle_name":null,"organization":"Broad Institute of MIT and Harvard","street":null,"town_or_city":"Cambridge","state_or_province":null,"post_or_zip":"02142","telephone":null,"fax":null,"first_name":"Feng","last_name":"Zhang","email":"zhang [at] broadinstitute.org","country":"United States","entry":"EMPIAR-11458"}],"status":"REL","deposition_date":"2023-01-25","release_date":"2023-04-11","obsolete_date":null,"update_date":"2023-04-11","corresponding_author":{"author":{"author_orcid":"0000-0003-4738-9503","middle_name":null,"organization":"Broad Institute of MIT and Harvard","street":"75 Ames St","town_or_city":"Cambridge","state_or_province":"Massachusetts","post_or_zip":"02142","first_name":"Max","last_name":"Wilkinson","country":"United States"}},"authors":[{"author":{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"}},{"author":{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}}],"cross_references":["EMD-40033"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Wilkinson ME","author_orcid":"0000-0003-4738-9503"},{"name":"Frangieh CJ","author_orcid":null},{"name":"Macrae RK","author_orcid":"0000-0002-7850-9056"},{"name":"Zhang F","author_orcid":"0000-0003-0178-7995"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription","volume":null,"country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1126/science.adg7883","pubmedid":"37023171","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science (New York, N.Y.)","journal_abbreviation":"Science","issue":null,"preprint":false}],"dataset_size":"2.3 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8gh6"],"entry_doi":"10.6019/EMPIAR-11458"}}