{"EMPIAR-11457":{"imagesets":[{"segmentations":[],"name":"Cryo-electron tomography of ChAdOx spikes 2Pro mutant in situ","directory":"data/tilt-series","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":142,"frames_per_image":41,"frame_range_min":35,"frame_range_max":41,"voxel_type":"32 BIT FLOAT","pixel_width":2.18,"pixel_height":2.18,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Tilt-series were acquired using ThermoFisher Titan Krios microscopes operated at 300 keV, equipped with a K3 camera and Quantum energy filter in zeros-loss mode. The energy slit width was set to 20 eV. The tilt-series were collected with serialEM using a dose-symmetric scheme, starting from 0° with a 3° tilt increment by a group of 3, with an angular range from -60° to 60°. The accumulated exposure for each tilt-series was ~120 e-/Å2, with a defocus range between -2 and -5 µm. The micrographs have been motion-corrected and stacked using IMOD newstack command. The dataset contains the motion-corrected tilt-series, transformation matrix file (*.xf), local transformation file (*local), tilt angle (*tlt) and gold beads positions (*erase), which are generated from IMOD.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-electron tomography of ChAdOx spikes (AZD2816)","principal_investigator":[{"author_orcid":"0000-0003-1803-691X","middle_name":null,"organization":"The University of Oxford","street":null,"town_or_city":"Oxford","state_or_province":null,"post_or_zip":"OX3 7BN","telephone":null,"fax":null,"first_name":"Peijun","last_name":"Zhang","email":"peijun.zhang [at] strubi.ox.ac.uk","country":"United Kingdom","entry":"EMPIAR-11457"}],"status":"REL","deposition_date":"2023-03-10","release_date":"2023-04-26","obsolete_date":null,"update_date":"2023-05-16","corresponding_author":{"author":{"author_orcid":"0000-0001-7268-0306","middle_name":null,"organization":"The University of Hong Kong","street":null,"town_or_city":"Hong Kong","state_or_province":null,"post_or_zip":"0000","first_name":"Tao","last_name":"Ni","country":"Hong Kong"}},"authors":[{"author":{"name":"Ni T","author_orcid":"0000-0001-7268-0306"}},{"author":{"name":"Mendonca L","author_orcid":"0000-0002-9210-9139"}},{"author":{"name":"Zhu Y","author_orcid":"0000-0003-1025-7929"}},{"author":{"name":"Howe A","author_orcid":null}},{"author":{"name":"Radecke J","author_orcid":null}},{"author":{"name":"Sheng Y","author_orcid":null}},{"author":{"name":"Krebs AS","author_orcid":null}},{"author":{"name":"Shah P","author_orcid":null}},{"author":{"name":"Allen E","author_orcid":null}},{"author":{"name":"Spencer A","author_orcid":null}},{"author":{"name":"Morris S","author_orcid":null}},{"author":{"name":"Stuart D","author_orcid":null}},{"author":{"name":"Gilbert S","author_orcid":null}},{"author":{"name":"Zhang P","author_orcid":"0000-0003-1803-691X"}}],"cross_references":["EMD-16406","EMD-16405"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Ni T","author_orcid":"0000-0001-7268-0306"},{"name":"Menconca L","author_orcid":"0000-0002-9210-9139"},{"name":"Zhu Y","author_orcid":"0000-0003-1025-7929"},{"name":"Howe A","author_orcid":"0000-0003-2187-7672"},{"name":"Radecke J","author_orcid":"0000-0002-5815-5537"},{"name":"Zhang P","author_orcid":"0000-0003-1803-691X"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"In situ structures of ChAdOx vaccine products reveal native prefusion spikes improved by HexaPro mutations","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":null,"pubmedid":null,"details":"The tilt-series have been motion corrected. The transformation files, tilt angle and gold beads positions are generated from Etomo. Tilt-series were acquired using ThermoFisher Titan Krios microscopes operated at 300 keV, equipped with a K3 camera and Quantum energy filter in zeros-loss mode. The energy slit width was set to 20 eV. The tilt-series were collected with serialEM using a dose-symmetric scheme, starting from 0° with a 3° tilt increment by a group of 3, with an angular range from -60° to 60°. The accumulated exposure for each tilt-series was ~120 e-/Å2, with a defocus range between -2 and -5 µm.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"494.4 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11457"}}