{"EMPIAR-11453":{"imagesets":[{"segmentations":[],"name":"Dataset 9 part 1: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset9_part1_221208/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":28,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":2.659,"pixel_height":2.659,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 9 part 1: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference is provided within the main folder. Data was collected on a K3 camera at a pixel size of 2.659 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.\nTS_330 - TS_351 are from cofilin knock-down cells. TS_352 - TS_366 are from control knock-down cells.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dataset 9 part 1: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset9_part1_221208/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":28,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.81,"pixel_height":11.81,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 9 part 1: Tomograms binned to 11.81 Å/ pixel, reconstructed and deconvolved in WARP.\nTS_330 - TS_351 are from cofilin knock-down cells. TS_352 - TS_366 are from control knock-down cells.","image_width":"920","image_height":"1298"},{"segmentations":[],"name":"Dataset 9 part 2: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset9_part2_221217/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":8,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 9 part 2: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.\nTS_462 - TS_470 are from control knock-down cells.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 9 part 2: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset9_part2_221217/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":8,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.81,"pixel_height":11.81,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 9 part 2: Tomograms binned by 4 (11.81 Å/ pixel), reconstructed and deconvolved in WARP.\nTS_462 - TS_470 are from control knock-down cells.","image_width":"960","image_height":"928"},{"segmentations":[],"name":"Dataset 10: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset10_221215/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":34,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 10: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.\nTS_424 - TS_442 are from cofilin knock-down cells. TS_443 - TS_461 are from control knock-down cells.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 10: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset10_221215/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":34,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.81,"pixel_height":11.81,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 10: Tomograms binned by 4 (11.81 Å/ pixel), reconstructed and deconvolved in WARP.\nTS_424 - TS_442 are from cofilin knock-down cells. TS_443 - TS_461 are from control knock-down cells.","image_width":"960","image_height":"928"},{"segmentations":[],"name":"Dataset 11 part 1: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset11_230202_part1/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":32,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 11 part 1: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.\nTS_495 - TS_505 are from cofilin knock-down cells. TS_506 - TS_527 are from control knock-down cells.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 11 part 1: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset11_230202_part1/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":32,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.8,"pixel_height":11.8,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 11 part 1: Tomograms binned by 4 (11.8 Å/ pixel), reconstructed and deconvolved in WARP.\nTS_495 - TS_505 are from cofilin knock-down cells. TS_506 - TS_527 are from control knock-down cells.","image_width":"960","image_height":"928"},{"segmentations":[],"name":"Dataset 11 part 2: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset11_221212_part2/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":3,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":2.659,"pixel_height":2.659,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 11 part 2: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference is provided within the main folder. Data was collected on a K3 camera at a pixel size of 2.659 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.\nTS_367, TS_371, TS_380 are from cofilin knock-down cells.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dataset 11 part 2: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset11_221212_part2/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":10.64,"pixel_height":10.64,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 11 part 2: Tomograms binned by 4 (10.64 Å/ pixel), reconstructed and deconvolved in WARP.\nTS_367, TS_371, TS_380 are from cofilin knock-down cells.","image_width":"1022","image_height":"1440"},{"segmentations":[],"name":"Dataset 12: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset12_230202/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":27,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 12: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.\nTS_528 - TS_540 are from cofilin knock-down cells. TS_542 - TS_556 are from control knock-down cells.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 12: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells","directory":"data/Dataset12_230202/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":27,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.8,"pixel_height":11.8,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 12: Tomograms binned by 4 (11.8 Å/ pixel), reconstructed and deconvolved in WARP.\nTS_528 - TS_540 are from cofilin knock-down cells. TS_542 - TS_556 are from control knock-down cells.","image_width":"960","image_height":"928"}],"workflow_file":null,"grant_references":[{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_UP_A025_1011","country":"United Kingdom"},{"funding_body":"Welch Foundation","code":"210711/Z/18/Z","country":"United Kingdom"}],"version_history":[],"title":"Cryo electron tomography of induced protrusions of cofilin or control knock-down Drosophila S2 cells - Datasets 9 - 12","principal_investigator":[{"author_orcid":"0000-0001-7292-5430","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Cambridge Biomedical Campus, Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Andrew","last_name":"Carter","email":"cartera [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-11453"}],"status":"REL","deposition_date":"2023-03-07","release_date":"2023-07-10","obsolete_date":null,"update_date":"2023-07-10","corresponding_author":{"author":{"author_orcid":"0000-0002-4406-5151","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Cambridge Biomedical Campus, Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","first_name":"Camilla","last_name":"Ventura Santos","country":"United Kingdom"}},"authors":[{"author":{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"}},{"author":{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"}},{"author":{"name":"Rogers SL","author_orcid":null}}],"cross_references":["EMD-16811","EMD-16800"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"},{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"},{"name":"Rogers SL","author_orcid":null}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"CryoET shows cofilactin filaments inside the microtubule lumen","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":null,"pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"491.9 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11453"}}