{"EMPIAR-11451":{"imagesets":[{"segmentations":[],"name":"Dataset 5: Raw multi-frame tilt images of Cytochalasin D and DMSO or thapsigarging treated Drosophila S2 cells","directory":"data/Dataset5_220617/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":24,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 5. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours and treated with either 0.1% DMSO (TS_201 - TS_214) or 2 uM thapsigargin (TS_215 - TS_228). The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) can be found within the Mdoc_files folder.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 5: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions","directory":"data/Dataset5_220617/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":24,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.81,"pixel_height":11.81,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 5: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP. Tomograms TS_201 - TS_214 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 0.1% DMSO (5 hours). TS_215 - TS_228 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 2 uM thapsigargin (5 hours).","image_width":"960","image_height":"928"},{"segmentations":[],"name":"Dataset 6: Raw multi-frame tilt images of Cytochalasin D and DMSO or thapsigarging treated Drosophila S2 cells","directory":"data/Dataset6_220720/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":33,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 6. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours and treated with either 0.1% DMSO (TS_262 - TS_284) or 2 uM thapsigargin (TS_153 - TS_161 and TS_243 - TS_251). The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) can be found within the Mdoc_files folder.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 6: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions","directory":"data/Dataset6_220720/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":33,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.81,"pixel_height":11.81,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 6: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP. \nTomograms TS_262 - TS_282 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 0.1% DMSO (5 hours). TS_153 - TS_161 and TS_243 - TS_251 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 2 uM thapsigargin (5 hours).","image_width":"960","image_height":"928"},{"segmentations":[],"name":"Dataset 7: Raw multi-frame tilt images of Cytochalasin D and DMSO or thapsigarging treated Drosophila S2 cells","directory":"data/Dataset7_220721/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":33,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":2.659,"pixel_height":2.659,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 7. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours and treated with either 0.1% DMSO (TS_304 - TS_328) or 2 uM thapsigargin (TS_285 - TS_303 and TS_329). The gain reference is provided within the folder. The pixel size is 2.659 Å/pixel. Data was collected on a K3 detector. Metadata from tilt series acquisition (Mdoc files) can be found within the Mdoc_files folder.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Dataset 7: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions","directory":"data/Dataset7_220721/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":33,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":10.64,"pixel_height":10.64,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 6: Tomograms binned by 4 (10.64 Å/pixel), reconstructed and deconvolved in WARP. \nTomograms TS_304 - TS_328 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 0.1% DMSO (5 hours). TS_285 - TS_303 and TS_329 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 2 uM thapsigargin (5 hours).","image_width":"1022","image_height":"1440"}],"workflow_file":null,"grant_references":[{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_UP_A025_1011","country":"United Kingdom"},{"funding_body":"Welch Foundation","code":"210711/Z/18/Z","country":"United Kingdom"}],"version_history":[],"title":"Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells treated with DMSO or thapsigargin - Datasets 5 - 7","principal_investigator":[{"author_orcid":"0000-0001-7292-5430","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Cambridge Biomedical Campus, Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Andrew","last_name":"Carter","email":"cartera [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-11451"}],"status":"REL","deposition_date":"2023-02-17","release_date":"2023-07-10","obsolete_date":null,"update_date":"2023-07-10","corresponding_author":{"author":{"author_orcid":"0000-0002-4406-5151","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Cambridge Biomedical Campus, Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","first_name":"Camilla","last_name":"Ventura Santos","country":"United Kingdom"}},"authors":[{"author":{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"}},{"author":{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"}},{"author":{"name":"Rogers SL","author_orcid":null}}],"cross_references":["EMD-16720"],"biostudies_references":[],"idr_references":[],"empiar_references":[{"name":"EMPIAR-11450"}],"citation":[{"authors":[{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"},{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"},{"name":"Rogers SL","author_orcid":null}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"CryoET shows cofilactin filaments inside the microtubule lumen","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":null,"pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"373.1 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11451"}}