{"EMPIAR-11450":{"imagesets":[{"segmentations":[],"name":"Dataset 1: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells","directory":"data/Dataset1_210428/Raw_Movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":25,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 1. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 1: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions","directory":"data/Dataset1_210428/bin4_deconvoled_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":25,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.808,"pixel_height":11.808,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 1: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.","image_width":"1124","image_height":"1124"},{"segmentations":[],"name":"Dataset 2: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells","directory":"data/Dataset2_210605DY2/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":17,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 2. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit.  Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 2: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions","directory":"data/Dataset2_210605DY2/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":17,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.808,"pixel_height":11.808,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 2: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.","image_width":"960","image_height":"928"},{"segmentations":[],"name":"Dataset 3: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells","directory":"data/Dataset3_210725_DZ1/Raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":39,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 3. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit.  Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 3: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions","directory":"data/Dataset3_210725_DZ1/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":39,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.808,"pixel_height":11.808,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 3: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.","image_width":"960","image_height":"928"},{"segmentations":[],"name":"Dataset 4: Raw multi-frame tilt images of Cytochalasin D induced protrusions of Drosophila S2 cells","directory":"data/Dataset4_210724FB34/raw_movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":22,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED 16 BIT INTEGER","pixel_width":2.952,"pixel_height":2.952,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data from Dataset 4. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 5 uM Cytochalasin D for 4 hours. The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit.  Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc folder.","image_width":"3710","image_height":"3838"},{"segmentations":[],"name":"Dataset 4: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions","directory":"data/Dataset4_210724FB34/bin4_deconvolved_tomograms","category":"reconstructed volumes","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":22,"frames_per_image":14,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":11.808,"pixel_height":11.808,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Dataset 4: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP.","image_width":"960","image_height":"928"}],"workflow_file":null,"grant_references":[{"funding_body":"Medical Research Council (MRC, United Kingdom)","code":"MC_UP_A025_1011","country":"United Kingdom"},{"funding_body":"Welch Foundation","code":"210711/Z/18/Z","country":"United Kingdom"}],"version_history":[],"title":"Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells - Datasets 1 - 4","principal_investigator":[{"author_orcid":"0000-0001-7292-5430","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Cambridge Biomedical Campus, Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","telephone":null,"fax":null,"first_name":"Andrew","last_name":"Carter","email":"cartera [at] mrc-lmb.cam.ac.uk","country":"United Kingdom","entry":"EMPIAR-11450"}],"status":"REL","deposition_date":"2023-02-10","release_date":"2023-07-10","obsolete_date":null,"update_date":"2023-07-10","corresponding_author":{"author":{"author_orcid":"0000-0002-4406-5151","middle_name":null,"organization":"MRC Laboratory of Molecular Biology","street":"Cambridge Biomedical Campus, Francis Crick Ave","town_or_city":"Cambridge","state_or_province":"Cambridgeshire","post_or_zip":"CB2 0QH","first_name":"Camilla","last_name":"Ventura Santos","country":"United Kingdom"}},"authors":[{"author":{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"}},{"author":{"name":"Carter AP","author_orcid":"0000-0001-7292-5430"}},{"author":{"name":"Rogers SL","author_orcid":null}}],"cross_references":["EMD-16685","EMD-16693"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Ventura Santos C","author_orcid":"0000-0002-4406-5151"},{"name":"Rogers SL","author_orcid":null},{"name":"Carter AP","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"CryoET shows cofilactin filaments inside the microtubule lumen","volume":null,"country":"","first_page":null,"last_page":null,"year":"2023","language":null,"doi":"10.1101/2023.03.31.535077","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"446.0 GB","experiment_type":"EMDB","scale":"cell","entry_doi":"10.6019/EMPIAR-11450"}}