{"EMPIAR-11407":{"imagesets":[{"segmentations":[],"name":"extracted particles - IgG3-C1-C4b complexes bound to DNP representing liposomes","directory":"data","category":"picked particles - multiframe - processed","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2561,"frames_per_image":10,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":3.48,"pixel_height":3.48,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"data","details":"Tilt series were collected on a Talos Arctica (200kV) microscope with a K3 direct electron detector and a Bioquantum energy filter (slit width=20 eV; Gatan). Tomograms were reconstructed using weighed back-projection in IMOD4.11.Particles were manually picked in EMAN2 (version 2.91) using 2spt_boxer_old.py. 2,561 particles were extracted from 101 tomograms with a box size of 256 pixels and a final pixel size of 3.48 Å/pixel.","image_width":"256","image_height":"256"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"IgG3-C1-C4b complex on a lipid bilayer","principal_investigator":[{"author_orcid":"0000-0002-1990-2333","middle_name":"H.","organization":"Department of Cell and Chemical Biology, Leiden University Medical Centre","street":"Einthovenweg 20","town_or_city":"Leiden","state_or_province":"Zuid Holland","post_or_zip":"2333 ZC","telephone":null,"fax":null,"first_name":"Thomas","last_name":"Sharp","email":"t.sharp [at] lumc.nl","country":"Netherlands","entry":"EMPIAR-11407"}],"status":"REL","deposition_date":"2023-01-31","release_date":"2023-06-23","obsolete_date":null,"update_date":"2023-06-23","corresponding_author":{"author":{"author_orcid":"0000-0002-1990-2333","middle_name":"H.","organization":"Department of Cell and Chemical Biology, Leiden University Medical Centre","street":"Einthovenweg 20","town_or_city":"Leiden","state_or_province":"Zuid Holland","post_or_zip":"2333 ZC","first_name":"Thomas","last_name":"Sharp","country":"Netherlands"}},"authors":[{"author":{"name":"Abendstein LA","author_orcid":"0000-0001-7634-5353"}},{"author":{"name":"Sharp THS","author_orcid":"0000-0002-1990-2333"}}],"cross_references":["EMD-16241","EMD-16250","EMD-16251"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Abendstein LA","author_orcid":"0000-0001-7634-5353"}],"editors":[],"published":false,"j_or_nj_citation":true,"title":"Complement is activated by elevated IgG3 hexameric platforms and deposits C4b onto distinct antibody domains","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":null,"pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"","journal_abbreviation":"","issue":null,"preprint":false}],"dataset_size":"160.1 GB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-11407"}}