{"EMPIAR-11405":{"imagesets":[{"segmentations":[],"name":"Unaligned micrographs of the ribosome/Sec61/KZR-8445 complex","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":30260,"frames_per_image":50,"frame_range_min":1,"frame_range_max":50,"voxel_type":"UNSIGNED BYTE","pixel_width":0.415,"pixel_height":0.415,"micrographs_file_pattern":"data/KZR445_raw_mics*/FoilHole*.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"The micrographs are the average, without any realignment of 50 movie frames (accumulating 47.66 electrons per square Ångstrom). Data was collected in super resolution mode (physical pixel size 0.83 px/Å) and binned 2x during preprocessing. Data collected on a Krios instrument with a K3 detector operated at 300 kV and at a nominal magnification of 105000x.\n\nDetails:\nNominal Defocus range (min, max, step): -0.6, -2.2\nTotal exposure on specimen: 47.66 e/A^2 \nExposure time: 3.0 s\nNominal pixel size: 0.83 A (0.415 A super res)\nObjective lens spherical aberration (Cs): 2.7 mm\nAcceleration voltage: 300 kV\nMicroscope / Detector: 300 kV FEI Titan Krios TEM / Gatan K3 summit","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Motion corrected micrographs of the ribosome/Sec61/KZR-8445 complex","directory":"data","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":30260,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/Motion_corrected_CTF*/KZR445_raw_mics*.mrc","picked_particles_file_pattern":"data/relion_model.star","picked_particles_directory":"data/","details":"Positions of particles for the final reconstructed volume in associated .star file.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[{"funding_body":"Academy of Finland","code":"338836","country":"Finland"},{"funding_body":"Academy of Finland","code":"314672","country":"Finland"},{"funding_body":"Academy of Finland","code":"314669","country":"Finland"}],"version_history":[],"title":"Cryo-EM structure of the ribosome/Sec61 translocon with a macrocyclic inhibitor KZR-8445","principal_investigator":[{"author_orcid":"0000-0002-3160-7767","middle_name":null,"organization":"HiLife Institute of Biotechnology, University of Helsinki","street":null,"town_or_city":"Helsinki","state_or_province":null,"post_or_zip":"00790","telephone":null,"fax":null,"first_name":"Ville","last_name":"Pavilainen","email":"ville.paavilainen [at] helsinki.fi","country":"Finland","entry":"EMPIAR-11405"}],"status":"REL","deposition_date":"2023-01-13","release_date":"2023-03-27","obsolete_date":null,"update_date":"2023-03-28","corresponding_author":{"author":{"author_orcid":"0000-0003-1329-8056","middle_name":null,"organization":"Omass Therapeutics","street":"The Oxford Science Park, The Schrödinger Building, Heatley Rd","town_or_city":"Oxford","state_or_province":"Oxfordshire","post_or_zip":"ox44sy","first_name":"Shahid","last_name":"Rehan","country":"United Kingdom"}},"authors":[{"author":{"name":"Rehan S","author_orcid":"0000-0003-1329-8056"}}],"cross_references":["EMD-14776"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rehan S","author_orcid":"0000-0003-1329-8056"},{"name":"Tranter D","author_orcid":null},{"name":"Sharp PP","author_orcid":null},{"name":"Lowe E","author_orcid":null},{"name":"Anderl JL","author_orcid":null},{"name":"Muchamuel T","author_orcid":null},{"name":"Abrishami V","author_orcid":null},{"name":"Kuivanen S","author_orcid":null},{"name":"Wenzell N","author_orcid":null},{"name":"Jennings A","author_orcid":null},{"name":"Kalyanaraman C","author_orcid":null},{"name":"Craven GB","author_orcid":null},{"name":"Strandin T","author_orcid":null},{"name":"Javanainen M","author_orcid":null},{"name":"Vapalahti O","author_orcid":null},{"name":"Jacobson M","author_orcid":null},{"name":"McMinn D","author_orcid":null},{"name":"Kirk CJ","author_orcid":null},{"name":"Huiskonen JT","author_orcid":null},{"name":"Taunton J","author_orcid":null},{"name":"Paavilainen VO","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Signal peptide mimicry primes Sec61 for client-selective inhibition","volume":null,"country":"","first_page":null,"last_page":null,"year":"2022","language":null,"doi":"10.1101/2022.07.03.498529","pubmedid":null,"details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"12.9 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7zl3"],"entry_doi":"10.6019/EMPIAR-11405"}}