{"EMPIAR-11397":{"imagesets":[{"segmentations":[],"name":"Aligned and dose weighted micrographs of mouse apoferritin sorted by ice thickness collected on a 200 kV Glacios microscope with a Falcon3 detector in integrating mode","directory":"data/22feb15b-Glacios_Falcon3_integrating","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3474,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.204,"pixel_height":1.204,"micrographs_file_pattern":"data/22feb15b-Glacios_Falcon3_integrating/*to*/*enn-a-DW.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs were aligned and dose weighted in MotionCor2. Here, they have been sorted into their respective ice thickness bins, 0-50 nm, 50-100 nm, 100-150 nm, 150-200 nm, and 200-500 nm. The obj_icegraph.txt file lists all micrographs collected during the session and the corresponding ice thickness measured by aperture limited scattering.","image_width":"4096","image_height":"4096"},{"segmentations":[],"name":"Aligned and dose weighted micrographs of mouse apoferritin sorted by energy filtered state and ice thickness collected on a 300 kV Krios microscope with a K3 detector in counting mode","directory":"data/22may20b-Krios_K3","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":14803,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.083,"pixel_height":1.083,"micrographs_file_pattern":"data/22may20b-Krios_K3/*filter*/*enn-a-DW.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs were aligned and dose weighted in MotionCor2. Here, they have been sorted into energy filtered state (with or without 20 eV energy filter) and respective ice thickness bins, 0-50 nm, 50-100 nm, 100-150 nm, 150-200 nm, and 200-500 nm. The zlp_icegraph.txt file lists all micrographs collected during the session and the corresponding ice thickness measured by energy filtration.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Aligned and dose weighted micrographs of mouse apoferritin collected on a 200 kV Arctica microscope with a K3 detector sorted by counting or super resolution mode and ice thickness","directory":"data/22sep21a-22sep22a-Arctica_K3","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":8666,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.096,"pixel_height":1.096,"micrographs_file_pattern":"data/22sep21a-22sep22a-Arctica_K3/*/*to*/*enn-a-DW.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Micrographs were aligned and dose weighted in MotionCor2. Here, they have been sorted into detector state (counting or super resolution) and respective ice thickness bins, 0-50 nm, 50-100 nm, 100-150 nm, 150-200 nm, and 200-500 nm. The obj_icegraph.txt or icethickness.txt file lists all micrographs collected during the session and the corresponding ice thickness measured by aperture limited scattering.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Raw movie frames of mouse apoferritin collected on a 200 kV Arctica microscope with a K3 detector in counting mode","directory":"data/22sep21a-22sep22a-Arctica_K3/counting/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5781,"frames_per_image":48,"frame_range_min":1,"frame_range_max":48,"voxel_type":"UNSIGNED BYTE","pixel_width":1.096,"pixel_height":1.096,"micrographs_file_pattern":"data/22sep21a-22sep22a-Arctica_K3/counting/frames/*enn.frames.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"These are the raw, unaligned movie frames. The obj_icegraph.txt file in the parent directory lists all micrographs collected during the session and the corresponding ice thickness measured by aperture limited scattering.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Raw movie frames of mouse apoferritin collected on a 200 kV Arctica microscope with a K3 detector in super resolution mode","directory":"data/22sep21a-22sep22a-Arctica_K3/superresolution/frames","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":2895,"frames_per_image":48,"frame_range_min":1,"frame_range_max":48,"voxel_type":"UNSIGNED BYTE","pixel_width":0.548,"pixel_height":0.548,"micrographs_file_pattern":"data/22sep21a-22sep22a-Arctica_K3/superresolution/frames/22sep22a_p8b6g1_00062sq_v02_00002hl_00002esn.frames.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"These are the raw, unaligned movie frames. The icethickness.txt file in the parent directory lists all micrographs collected during the session and the corresponding ice thickness measured by aperture limited scattering.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[{"funding_body":"Sigrid Juselius Foundation","code":"SF349247","country":"United States"},{"funding_body":"National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)","code":"GM103310","country":"United States"},{"funding_body":"Other government","code":"NYU Langone Health Cryo-EM core facility, RRID: SCR_019202","country":"United States"}],"version_history":[],"title":"CryoEM micrographs of mouse apoferritin in a range of ice thicknesses on different microscope setups","principal_investigator":[{"author_orcid":"0000-0002-0624-5020","middle_name":null,"organization":"New York Structural Biology Center","street":"89 Convent Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","telephone":null,"fax":null,"first_name":"Bridget","last_name":"Carragher","email":"bcarr [at] nysbc.org","country":"United States","entry":"EMPIAR-11397"},{"author_orcid":"0000-0003-2394-0831","middle_name":"S","organization":"New York Structural Biology Center","street":"89 Convent Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","telephone":null,"fax":null,"first_name":"Clinton","last_name":"Potter","email":"cpotter [at] nysbc.org","country":"United States","entry":"EMPIAR-11397"}],"status":"REL","deposition_date":"2022-11-30","release_date":"2023-02-27","obsolete_date":null,"update_date":"2023-02-27","corresponding_author":{"author":{"author_orcid":"0000-0003-1224-2157","middle_name":"YD","organization":"New York Structural Biology Center","street":"89 Convent Avenue","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","first_name":"Eugene","last_name":"Chua","country":"United States"}},"authors":[{"author":{"name":"Neselu K","author_orcid":"0000-0002-6908-9037"}},{"author":{"name":"Wang B","author_orcid":"0000-0002-9038-9878"}},{"author":{"name":"Rice WJ","author_orcid":"0000-0002-5187-255X"}},{"author":{"name":"Potter CS","author_orcid":"0000-0003-2394-0831"}},{"author":{"name":"Carragher B","author_orcid":"0000-0002-0624-5020"}},{"author":{"name":"Chua EYD","author_orcid":"0000-0003-1224-2157"}}],"cross_references":["EMD-29591","EMD-29594","EMD-29593","EMD-29589","EMD-29592","EMD-29513","EMD-29393","EMD-29555","EMD-29554","EMD-29559"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Neselu K","author_orcid":"0000-0002-6908-9037"},{"name":"Wang B","author_orcid":"0000-0002-9038-9878"},{"name":"Rice WJ","author_orcid":"0000-0002-5187-255X"},{"name":"Potter CS","author_orcid":"0000-0003-2394-0831"},{"name":"Carragher B","author_orcid":"0000-0002-0624-5020"},{"name":"Chua EYD","author_orcid":"0000-0003-1224-2157"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Measuring the effects of ice thickness on resolution in single particle cryo-EM","volume":"7","country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1016/j.yjsbx.2023.100085","pubmedid":"36742017","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Journal of structural biology: X","journal_abbreviation":"J Struct Biol X","issue":null,"preprint":false}],"dataset_size":"5.7 TB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-11397"}}