{"EMPIAR-11348":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of LiRecT in complex with 83-mer annealed duplex","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2038,"frames_per_image":36,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.899,"pixel_height":0.899,"micrographs_file_pattern":"data/*fractions.mrc","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Data collected on 9/17/2020-9/18-2020\nKrios, K3, counted mode, EFTEM, Cs corrector\nUser/Group:Brian Caldwell/Chuck Bell\n\nImage information:\nTotal # of images = 2038\nTotal data size = 1.70 TB\nRate of collection = 71/hour\nMode of collection = Accurate hole centering\nGain normalized = YES\nFile format = mrc\nSuper-resolution = NO \nSpherical aberration = 0.01 mm \nImages per hole = 1\nExposure time = 2.7 s\nFractions = 36 [usually 1-2 electrons per fractions]\n\nMicroscope parameters:\nPixel size = 0.899 A\nMagnification = 81,000\nStage tilt = 0 deg\nDose rate = 19.644 e/px/s\nDose rate = 24.28 e/A^2/s\nTotal dose = 65 e/A^2 [desired dose rate]\nDefocus range = -3.5, -3, -2.5, -2, -1.5, -1\nC2 aperture = 50 um\nObjective aperture= 100 um\nIlluminated area = 1.5 um\nEnergy Filter = YES\nSlit width = 20 eV\n\nGrid type = Quantifoil (gold coated) R1.2/1.3 300 Mesh hole size, mesh, etc.\nPosition 1 of the large dewar Bell Conical: Position 1 of box J2 (Gray)\nPrepared on 8/20/2020 by Brian Caldwell\n\nAdditional notes:\nCopied gain reference files over into the data folder.\nSaved EPU settings file.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Structure of RecT protein from Listeria innoccua phage A118 in complex with 83-mer annealed duplex","principal_investigator":[{"author_orcid":"0000-0001-6486-9408","middle_name":"E","organization":"Department of Biological Chemistry and Pharmacology, Ohio State University","street":"1060 Carmack Rd","town_or_city":"Columbus","state_or_province":"Ohio","post_or_zip":"43210","telephone":"+1 6143233225","fax":"+1 6142924118","first_name":"Charles","last_name":"Bell","email":"bell.489 [at] osu.edu","country":"United States","entry":"EMPIAR-11348"}],"status":"REL","deposition_date":"2022-03-15","release_date":"2023-01-30","obsolete_date":null,"update_date":"2023-01-30","corresponding_author":{"author":{"author_orcid":"0000-0001-6486-9408","middle_name":"E","organization":"Department of Biological Chemistry and Pharmacology, Ohio State University","street":"1060 Carmack Rd","town_or_city":"Columbus","state_or_province":"Ohio","post_or_zip":"43210","first_name":"Charles","last_name":"Bell","country":"United States"}},"authors":[{"author":{"name":"Bell CE","author_orcid":"0000-0001-6486-9408"}}],"cross_references":["EMD-26434"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Caldwell BJ","author_orcid":null},{"name":"Norris AS","author_orcid":null},{"name":"Karbowski CF","author_orcid":"0000-0002-1522-3333"},{"name":"Wiegand AM","author_orcid":null},{"name":"Wysocki VH","author_orcid":"0000-0003-0495-2538"},{"name":"Bell CE","author_orcid":"0000-0001-6486-9408"}],"editors":[{"name":" ","author_orcid":null}],"published":true,"j_or_nj_citation":true,"title":"Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing","volume":"13","country":"","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.1038/s41467-022-35572-z","pubmedid":"36543802","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"1.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7ub2"],"entry_doi":"10.6019/EMPIAR-11348"}}