{"EMPIAR-11338":{"imagesets":[{"segmentations":[],"name":"K3 Movies (.tif) for SKV09 Antibody + VEEV VLP","directory":"data/VEEV_SKV09/movies","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5595,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/VEEV_SKV09/movies/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Cryo-EM data for single particle analysis were collected at the Columbia Cryo-EM Facility on a Titan Krios electron microscope operating at 300 kV, equipped with a Gatan K3-BioQuantum direct detection detector and energy filter, using the Leginon software package. Exposures were taken at a magnification of 105,000x (pixel size of 0.83 Å), using a total electron fluence of 46.5 e-/Å2 fractionated over 40 frames with an exposure time of 2.0 s. A random defocus range of -0.8 to -2.0 µm was used.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM SPA dataset for Antibody SKV09 in complex with VEEV VLP (K3 movies/.tif files)","principal_investigator":[{"author_orcid":"0000-0001-9943-8819","middle_name":null,"organization":"Department of Biochemistry, Columbia University","street":"3227 Broadway","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","telephone":null,"fax":null,"first_name":"Lawrence","last_name":"Shapiro","email":"lawrenceshapiro [at] gmail.com","country":"United States","entry":"EMPIAR-11338"}],"status":"REL","deposition_date":"2022-12-02","release_date":"2023-07-07","obsolete_date":null,"update_date":"2023-07-07","corresponding_author":{"author":{"author_orcid":"0000-0002-2910-0908","middle_name":"Gavin","organization":"Columbia University","street":"3227 Broadway","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","first_name":"Ryan","last_name":"Casner","country":"United States"}},"authors":[{"author":{"name":"Casner RG","author_orcid":"0000-0002-2910-0908"}}],"cross_references":["EMD-28187","EMD-27395"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Sutton MS","author_orcid":null},{"name":"Pletnev S","author_orcid":null},{"name":"Callahan V","author_orcid":null},{"name":"Ko S","author_orcid":null},{"name":"Tsybovsky Y","author_orcid":null},{"name":"Bylund T","author_orcid":null},{"name":"Casner RG","author_orcid":"0000-0002-2910-0908"},{"name":"Cerutti G","author_orcid":null},{"name":"Gardner CL","author_orcid":null},{"name":"Guirguis V","author_orcid":null},{"name":"Verardi R","author_orcid":null},{"name":"Zhang B","author_orcid":null},{"name":"Ambrozak D","author_orcid":null},{"name":"Beddall M","author_orcid":null},{"name":"Lei H","author_orcid":null},{"name":"Yang ES","author_orcid":null},{"name":"Liu T","author_orcid":null},{"name":"Henry AR","author_orcid":null},{"name":"Rawi R","author_orcid":null},{"name":"Schön A","author_orcid":null},{"name":"Schramm CA","author_orcid":null},{"name":"Shen CH","author_orcid":null},{"name":"Shi W","author_orcid":null},{"name":"Stephens T","author_orcid":null},{"name":"Yang Y","author_orcid":null},{"name":"Florez MB","author_orcid":null},{"name":"Ledgerwood JE","author_orcid":null},{"name":"Burke CW","author_orcid":null},{"name":"Shapiro L","author_orcid":null},{"name":"Fox JM","author_orcid":null},{"name":"Kwong PD","author_orcid":null},{"name":"Roederer M","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Vaccine elicitation and structural basis for antibody protection against alphaviruses","volume":"186","country":"","first_page":"2672","last_page":"2689.e25","year":"2023","language":"English","doi":"10.1016/j.cell.2023.05.019","pubmedid":"37295404","details":"Cryo-EM data for single particle analysis were collected at the Columbia Cryo-EM Facility on a Titan Krios electron microscope operating at 300 kV, equipped with a Gatan K3-BioQuantum direct detection detector and energy filter, using the Leginon software package. Exposures were taken at a magnification of 105,000x (pixel size of 0.83 Å), using a total electron fluence of 46.5 e-/Å2 fractionated over 40 frames with an exposure time of 2.0 s. A random defocus range of -0.8 to -2.0 µm was used.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Cell","journal_abbreviation":"Cell","issue":"12","preprint":false}],"dataset_size":"776.7 GB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8deq"],"entry_doi":"10.6019/EMPIAR-11338"}}