{"EMPIAR-11335":{"imagesets":[{"segmentations":[],"name":"K3 Movies (.tif) for 12-16 Antibody + Spike","directory":"data/12-16","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4088,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/12-16/day1/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Collected on a Krios using a K3 BioQuantum.\nThe gain reference is in data/12-16/day1/references/*norm_0.mrc.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"K3 Movies (.tif) for 12-19 Antibody + Spike","directory":"data/12-19","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":7099,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.83,"pixel_height":0.83,"micrographs_file_pattern":"data/12-19/day?/*.tif","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Collected on a Krios using a K3 BioQuantum.\nday 1 has thinner ice and less particles.\nday 2 has thicker ice and more particles.\nThe gain reference is in data/12-19/day2/references/*norm_0.mrc.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM SPA datasets for Antibodies 12-16 & 12-19 in complex with prefusion SARS-CoV-2 Spike glycoprotein (K3 movies/.tif files)","principal_investigator":[{"author_orcid":"0000-0001-9943-8819","middle_name":null,"organization":"Department of Biochemistry, Columbia University","street":"3227 Broadway","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","telephone":null,"fax":null,"first_name":"Lawrence","last_name":"Shapiro","email":"lss8 [at] columbia.edu","country":"United States","entry":"EMPIAR-11335"}],"status":"REL","deposition_date":"2022-12-02","release_date":"2023-10-09","obsolete_date":null,"update_date":"2023-10-09","corresponding_author":{"author":{"author_orcid":"0000-0002-2910-0908","middle_name":"Gavin","organization":"Columbia University","street":"3227 Broadway","town_or_city":"New York","state_or_province":"NY","post_or_zip":"10027","first_name":"Ryan","last_name":"Casner","country":"United States"}},"authors":[{"author":{"name":"Casner RG","author_orcid":"0000-0002-2910-0908"}},{"author":{"name":"Shapiro L","author_orcid":"0000-0001-9943-8819"}}],"cross_references":["EMD-26584","EMD-26583"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Liu L","author_orcid":null},{"name":"Casner RG","author_orcid":"0000-0002-2910-0908"},{"name":"Guo Y","author_orcid":null},{"name":"Wang Q","author_orcid":null},{"name":"Iketani S","author_orcid":null},{"name":"Chan JF","author_orcid":null},{"name":"Yu J","author_orcid":null},{"name":"Dadonaite B","author_orcid":null},{"name":"Nair MS","author_orcid":null},{"name":"Mohri H","author_orcid":null},{"name":"Reddem ER","author_orcid":null},{"name":"Yuan S","author_orcid":null},{"name":"Poon VK","author_orcid":null},{"name":"Chan CC","author_orcid":null},{"name":"Yuen KY","author_orcid":null},{"name":"Sheng Z","author_orcid":null},{"name":"Huang Y","author_orcid":null},{"name":"Bloom JD","author_orcid":null},{"name":"Shapiro L","author_orcid":"0000-0001-9943-8819"},{"name":"Ho DD","author_orcid":null}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Antibodies targeting a quaternary site on SARS-CoV-2 spike glycoprotein prevent viral receptor engagement by conformational locking","volume":null,"country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1016/j.immuni.2023.09.003","pubmedid":"37776849","details":"Cryo-EM data for single particle analysis were collected at the Columbia Cryo-EM Facility on a Titan Krios electron microscope operating at 300 kV, equipped with a Gatan K3-BioQuantum direct detection detector and energy filter, using the Leginon software package. Exposures were taken at a magnification of 105,000x (pixel size of 0.83 Å), using a total electron fluence of 58 e-/Å2 fractionated over 50 frames with an exposure time of 2.5 s. A random defocus range of -0.8 to -2.0 µm was used.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Immunity","journal_abbreviation":"Immunity","issue":null,"preprint":false}],"dataset_size":"2.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7ukm","7ukl"],"entry_doi":"10.6019/EMPIAR-11335"}}