{"EMPIAR-11327":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of phage T5 tails pre-incubated with bacteria receptor FhuA inserted in nanodiscs","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":5733,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.351,"pixel_height":1.351,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Purified tails from bacteriophage T5 after interaction with E. coli receptor FhuA inserted into nanodiscs - dataset 2","principal_investigator":[{"author_orcid":"0000-0001-9226-3382","middle_name":null,"organization":"Institut de Biologie Structurale","street":"71 avenue des Martyrs","town_or_city":"GRENOBLE","state_or_province":null,"post_or_zip":"38044 CEDEX 9","telephone":null,"fax":null,"first_name":"Romain","last_name":"Linares","email":"romain.linares [at] ibs.fr","country":"France","entry":"EMPIAR-11327"}],"status":"REL","deposition_date":"2022-11-22","release_date":"2024-02-01","obsolete_date":null,"update_date":"2024-02-01","corresponding_author":{"author":{"author_orcid":"0000-0001-9226-3382","middle_name":null,"organization":"Institut de Biologie Structurale","street":"71 avenue des martyrs","town_or_city":"GRENOBLE","state_or_province":"-","post_or_zip":"38044 CEDEX9","first_name":"Romain","last_name":"Linares","country":"France"}},"authors":[{"author":{"name":"Linares R","author_orcid":"0000-0001-9226-3382"}},{"author":{"name":"Arnaud C","author_orcid":null}},{"author":{"name":"Effantin G","author_orcid":"0000-0002-6957-0875"}},{"author":{"name":"Darnault C","author_orcid":"0000-0001-7777-2381"}},{"author":{"name":"Epalle NH","author_orcid":null}},{"author":{"name":"Erba EB","author_orcid":"0000-0002-5910-4707"}},{"author":{"name":"Schoehn G","author_orcid":"0000-0002-1459-3201"}},{"author":{"name":"Breyton C","author_orcid":"0000-0002-4382-0434"}}],"cross_references":["EMD-14873","EMD-14799","EMD-14800","EMD-13953"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Linares R","author_orcid":"0000-0001-9226-3382"},{"name":"Arnaud C","author_orcid":null},{"name":"Effantin G","author_orcid":"0000-0002-6957-0875"},{"name":"Darnault C","author_orcid":"0000-0001-7777-2381"},{"name":"Epalle NH","author_orcid":null},{"name":"Erba EB","author_orcid":"0000-0002-5910-4707"},{"name":"Schoehn G","author_orcid":"0000-0002-1459-3201"},{"name":"Breyton C","author_orcid":"0000-0002-4382-0434"}],"editors":[{"name":" ","author_orcid":null}],"published":true,"j_or_nj_citation":true,"title":"Structural basis of bacteriophage T5 infection trigger and <i>E. coli</i> cell wall perforation","volume":null,"country":"","first_page":null,"last_page":null,"year":"2022","language":null,"doi":"10.1101/2022.09.20.507954","pubmedid":null,"details":"This is a cryo-EM sample of purified tails from bacteriophage T5, after incubation with E.coli bacterial receptor FhuA inserted into nanodiscs.\nThis is the second of two separate data collections done on this sample.\n\nTypically, 3.5 µL of T5 tail incubated with FhuA-nanodisc sample were deposited on a freshly glow discharged (25 mA, 30 sec) Cu/Rh 300 mesh Quantifoil R 2/1 EM grids and plunge frozen in nitrogen-cooled liquid ethane using a ThermoFisher Mark IV Vitrobot device (100% humidity, 20°C, 5s blotting time, blot force 0)\n\nThe dataset contains 5733 40-frame movie, acquired on a Thermo Fisher Scientific Titan Krios G3 TEM (European Synchrotron Radiation Facility, Grenoble, France) operated at 300 kV and equipped with a Gatan Quantum energy filter coupled to a Gatan K2 summit direct electron detector. Automated data collection was performed using Thermo Fisher Scientific EPU software, with a typical defocus range of -1.0 to -3.0 µm and a total dose of 40 e-/Å2 per movie. A nominal magnification of 105.000x was used, resulting in a calibrated pixel size at the specimen level of 1.351 Å.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true},{"authors":[{"name":"Linares R","author_orcid":"0000-0001-9226-3382"},{"name":"Arnaud CA","author_orcid":null},{"name":"Effantin G","author_orcid":"0000-0002-6957-0875"},{"name":"Darnault C","author_orcid":"0000-0001-7777-2381"},{"name":"Epalle NH","author_orcid":null},{"name":"Boeri Erba E","author_orcid":"0000-0002-5910-4707"},{"name":"Schoehn G","author_orcid":"0000-0002-1459-3201"},{"name":"Breyton C","author_orcid":"0000-0002-4382-0434"}],"editors":[{"name":" ","author_orcid":null}],"published":true,"j_or_nj_citation":true,"title":"Structural basis of bacteriophage T5 infection trigger and <i>E. coli</i> cell wall perforation","volume":"9","country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1126/sciadv.ade9674","pubmedid":"36961893","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"12","preprint":false}],"dataset_size":"3.0 TB","experiment_type":"EMDB","scale":"virus","related_pdb_entries":["7zqp","7zn2","7zn4","7qg9"],"entry_doi":"10.6019/EMPIAR-11327"}}