{"EMPIAR-11319":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of phage T5 tails","directory":"data","category":"micrographs - multiframe","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3208,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.351,"pixel_height":1.351,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"","image_width":"3838","image_height":"3710"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Purified tails from bacteriophage T5","principal_investigator":[{"author_orcid":"0000-0001-9226-3382","middle_name":null,"organization":"Institut de Biologie Structurale","street":"71 avenue des Martyrs","town_or_city":"GRENOBLE","state_or_province":null,"post_or_zip":"38044 CEDEX 9","telephone":null,"fax":null,"first_name":"Romain","last_name":"Linares","email":"romain.linares [at] ibs.fr","country":"France","entry":"EMPIAR-11319"}],"status":"REL","deposition_date":"2022-11-14","release_date":"2023-07-18","obsolete_date":null,"update_date":"2023-07-18","corresponding_author":{"author":{"author_orcid":"0000-0001-9226-3382","middle_name":null,"organization":"Institut de Biologie Structurale","street":"71 avenue des martyrs","town_or_city":"GRENOBLE","state_or_province":"-","post_or_zip":"38044 CEDEX9","first_name":"Romain","last_name":"Linares","country":"France"}},"authors":[{"author":{"name":"Linares R","author_orcid":"0000-0001-9226-3382"}},{"author":{"name":"Arnaud CA","author_orcid":null}},{"author":{"name":"Effantin G","author_orcid":"0000-0002-6957-0875"}},{"author":{"name":"Darnault C","author_orcid":"0000-0001-7777-2381"}},{"author":{"name":"Epalle NH","author_orcid":null}},{"author":{"name":"Boeri Erba E","author_orcid":"0000-0002-5910-4707"}},{"author":{"name":"Schoehn G","author_orcid":"0000-0002-1459-3201"}},{"author":{"name":"Breyton C","author_orcid":"0000-0002-4382-0434"}}],"cross_references":["EMD-14733","EMD-14790","EMD-14869","EMD-13953"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Linares R","author_orcid":"0000-0001-9226-3382"},{"name":"Arnaud CA","author_orcid":null},{"name":"Effantin G","author_orcid":"0000-0002-6957-0875"},{"name":"Darnault C","author_orcid":"0000-0001-7777-2381"},{"name":"Epalle NH","author_orcid":null},{"name":"Boeri Erba E","author_orcid":"0000-0002-5910-4707"},{"name":"Schoehn G","author_orcid":"0000-0002-1459-3201"},{"name":"Breyton C","author_orcid":"0000-0002-4382-0434"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis of bacteriophage T5 infection trigger and <i>E. coli</i> cell wall perforation","volume":"9","country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1126/sciadv.ade9674","pubmedid":"36961893","details":"This is a cryo-EM sample of purified tails from bacteriophage T5.\n\nTypically, 3.5 µL of T5 tail sample were deposited on a freshly glow discharged (25 mA, 30 sec) Cu/Rh 300 mesh Quantifoil R 2/1 EM grids and plunge frozen in nitrogen-cooled liquid ethane using a ThermoFisher Mark IV Vitrobot device (100% humidity, 20°C, 5s blotting time, blot force 0)\n\nThe dataset contains 3208 40-frame movie, acquired on a Thermo Fisher Scientific Titan Krios G3 TEM (European Synchrotron Radiation Facility, Grenoble, France) operated at 300 kV and equipped with a Gatan Quantum energy filter coupled to a Gatan K2 summit direct electron detector. Automated data collection was performed using Thermo Fisher Scientific EPU software, with a typical defocus range of -1.0 to -3.0 µm and a total dose of 40 e-/Å2 per movie. A nominal magnification of 105.000x was used, resulting in a calibrated pixel size at the specimen level of 1.351 Å.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"12","preprint":false},{"authors":[{"name":"Linares R","author_orcid":"0000-0001-9226-3382"},{"name":"Arnaud CA","author_orcid":null},{"name":"Effantin G","author_orcid":"0000-0002-6957-0875"},{"name":"Darnault C","author_orcid":"0000-0001-7777-2381"},{"name":"Epalle NH","author_orcid":null},{"name":"Boeri Erba E","author_orcid":"0000-0002-5910-4707"},{"name":"Schoehn G","author_orcid":"0000-0002-1459-3201"},{"name":"Breyton C","author_orcid":"0000-0002-4382-0434"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis of bacteriophage T5 infection trigger and E. coli cell wall perforation","volume":null,"country":"","first_page":null,"last_page":null,"year":null,"language":null,"doi":"10.1101/2022.09.20.507954","pubmedid":null,"details":"This is a cryo-EM sample of purified tails from bacteriophage T5.\n\nTypically, 3.5 µL of T5 tail sample were deposited on a freshly glow discharged (25 mA, 30 sec) Cu/Rh 300 mesh Quantifoil R 2/1 EM grids and plunge frozen in nitrogen-cooled liquid ethane using a ThermoFisher Mark IV Vitrobot device (100% humidity, 20°C, 5s blotting time, blot force 0)\n\nThe dataset contains 3208 40-frame movie, acquired on a Thermo Fisher Scientific Titan Krios G3 TEM (European Synchrotron Radiation Facility, Grenoble, France) operated at 300 kV and equipped with a Gatan Quantum energy filter coupled to a Gatan K2 summit direct electron detector. Automated data collection was performed using Thermo Fisher Scientific EPU software, with a typical defocus range of -1.0 to -3.0 µm and a total dose of 40 e-/Å2 per movie. A nominal magnification of 105.000x was used, resulting in a calibrated pixel size at the specimen level of 1.351 Å.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"BioRxiv","journal_abbreviation":"","issue":null,"preprint":true}],"dataset_size":"6.6 TB","experiment_type":"EMDB","scale":"virus","related_pdb_entries":["7zhj","7zlv","7zqb","7qg9"],"entry_doi":"10.6019/EMPIAR-11319"}}