{"EMPIAR-11313":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of Ccm1-bound yeast mtSSU assembly intermediates","directory":"data/Krios2_11_11_21/tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":31995,"frames_per_image":50,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 31,995 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of Rsm22-bound yeast mtSSU assembly intermediates","directory":"data/Krios2_3_19_21/tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":14111,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 14,111 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of METTL17-bound human mtSSU assembly intermediates (dataset 1)","directory":"data/tifmerge","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":37112,"frames_per_image":42,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 37,112 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Unaligned multi-frame micrographs of METTL17-bound human mtSSU assembly intermediates (dataset 2)","directory":"data/3x3tiffs","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":9990,"frames_per_image":42,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.54,"pixel_height":0.54,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 9,990 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.","image_width":"11520","image_height":"8184"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Principles of mitoribosomal small subunit assembly in eukaryotes","principal_investigator":[{"author_orcid":"0000-0002-9373-4737","middle_name":null,"organization":"The Rockefeller University","street":null,"town_or_city":"New York","state_or_province":null,"post_or_zip":"10065","telephone":null,"fax":null,"first_name":"Sebastian","last_name":"Klinge","email":"klinge [at] mail.rockefeller.edu","country":"United States","entry":"EMPIAR-11313"}],"status":"REL","deposition_date":"2022-10-21","release_date":"2023-02-17","obsolete_date":null,"update_date":"2023-02-17","corresponding_author":{"author":{"author_orcid":"0000-0002-7298-4841","middle_name":null,"organization":"The Rockefeller University","street":null,"town_or_city":"New York","state_or_province":null,"post_or_zip":"10065","first_name":"Chloe","last_name":"Burnside","country":"United States"}},"authors":[{"author":{"name":"Harper NJ","author_orcid":"0000-0001-5968-4046"}},{"author":{"name":"Burnside C","author_orcid":"0000-0002-7298-4841"}},{"author":{"name":"Klinge S","author_orcid":"0000-0002-9373-4737"}}],"cross_references":["EMD-27250","EMD-27249","EMD-26969","EMD-26967","EMD-26968","EMD-26970","EMD-26966","EMD-27251","EMD-26971"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Harper NJ","author_orcid":"0000-0001-5968-4046"},{"name":"Burnside C","author_orcid":"0000-0002-7298-4841"},{"name":"Klinge S","author_orcid":"0000-0002-9373-4737"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Principles of mitoribosomal small subunit assembly in eukaryotes","volume":"614","country":"","first_page":"175","last_page":"181","year":"2022","language":"English","doi":"10.1038/s41586-022-05621-0","pubmedid":"36482135","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature","journal_abbreviation":"Nature","issue":"7946","preprint":false}],"dataset_size":"73.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8d8k","8d8j","8css","8csq","8csr","8cst","8csp","8d8l","8csu"],"entry_doi":"10.6019/EMPIAR-11313"}}