{"EMPIAR-11311":{"imagesets":[{"segmentations":[],"name":"Unaligned movie frames (Dataset 1)","directory":"data/raw_220226_LdECF-2EQv2-MSP2N2-bCT63s5_ngc","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":9932,"frames_per_image":75,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.836,"pixel_height":0.836,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame movies with each containing 75 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 300 kV in super-resolution counting mode with hardware binning by a factor of 2 (Counted Super Resolution Bin 2). Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU and Aberration Free Image Shift (AFIS) enabled.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Unaligned movie frames (Dataset 2)","directory":"data/raw_220228_LdECF-2EQv2-MSP2N2-bCT63s3_ngc","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":5326,"frames_per_image":75,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":0.836,"pixel_height":0.836,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Unaligned multi-frame movies with each containing 75 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 300 kV in super-resolution counting mode with hardware binning by a factor of 2 (Counted Super Resolution Bin 2). Total accumulated electron exposure on the specimen level corresponds to 60 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU and Aberration Free Image Shift (AFIS) enabled.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-EM structure of the mutant solitary ECF module 2EQ in MSP2N2 lipid nanodiscs in the ATPase closed and ATP-bound conformation (300 kV)","principal_investigator":[{"author_orcid":"0000-0001-7017-109X","middle_name":null,"organization":"Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen","street":"Nijenborgh 4","town_or_city":"Groningen","state_or_province":null,"post_or_zip":"9747 AG","telephone":null,"fax":null,"first_name":"Cristina","last_name":"Paulino","email":"cpaulinolab [at] gmail.com","country":"Netherlands","entry":"EMPIAR-11311"},{"author_orcid":"0000-0002-5804-9689","middle_name":null,"organization":"Faculty of Science and Engineering, Membrane Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen","street":"Nijenborgh 4","town_or_city":"Groningen","state_or_province":null,"post_or_zip":"9747 AG","telephone":null,"fax":null,"first_name":"Dirk Jan","last_name":"Slotboom","email":"d.j.slotboom [at] rug.nl","country":"Netherlands","entry":"EMPIAR-11311"}],"status":"REL","deposition_date":"2022-11-15","release_date":"2023-09-06","obsolete_date":null,"update_date":"2023-09-06","corresponding_author":{"author":{"author_orcid":"0000-0001-7017-109X","middle_name":null,"organization":"Department of Biochemistry, Structural Biology Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen","street":"Nijenborgh 4","town_or_city":"Groningen","state_or_province":null,"post_or_zip":"9747 AG","first_name":"Cristina","last_name":"Paulino","country":"Netherlands"}},"authors":[{"author":{"name":"Thangaratnarajah C","author_orcid":"0000-0002-0279-6642"}},{"author":{"name":"Rheinberger J","author_orcid":"0000-0002-9901-2065"}},{"author":{"name":"Paulino C","author_orcid":"0000-0001-7017-109X"}},{"author":{"name":"Slotboom DJ","author_orcid":"0000-0002-5804-9689"}}],"cross_references":["EMD-16124"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Thangaratnarajah C","author_orcid":"0000-0002-0279-6642"},{"name":"Nijland M","author_orcid":null},{"name":"Borges-Araújo L","author_orcid":"0000-0003-3004-7750"},{"name":"Jeucken A","author_orcid":null},{"name":"Rheinberger J","author_orcid":"0000-0002-9901-2065"},{"name":"Marrink SJ","author_orcid":"0000-0001-8423-5277"},{"name":"Souza PCT","author_orcid":"0000-0003-0660-1301"},{"name":"Paulino C","author_orcid":"0000-0001-7017-109X"},{"name":"Slotboom DJ","author_orcid":"0000-0002-5804-9689"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Expulsion mechanism of the substrate-translocating subunit in ECF transporters","volume":"14","country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1038/s41467-023-40266-1","pubmedid":"37491368","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature communications","journal_abbreviation":"Nat Commun","issue":"1","preprint":false}],"dataset_size":"4.4 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["8bms"],"entry_doi":"10.6019/EMPIAR-11311"}}