{"EMPIAR-11305":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of reconstituted USP1, UAF1, FANCI, monoubiquitinated FANCD2, and DNA","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":4593,"frames_per_image":59,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.015,"pixel_height":1.015,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Multi-frame raw micrographs without gain correction in LZW compressed TIFF were generated from gain-corrected MRCs using relion_convert_to_tiff. Two batches of micrographs are uploaded (data/batch*/*.tif), each with a separate gain reference. Imaging was performed at 120,000x magnification on JEOL CryoARM 300 equipped with a DE64 detector at SCMI on 2020-08-17.\nSample contained 8.8 μM of each of USP1(C90S), UAF1, FANCI, FANCD2 monoubiquitinated on K561 and dsDNA (61 base pairs).","image_width":"4096","image_height":"4096"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Reconstituted USP1, UAF1, FANCI, and mono-ubiquitinated FANCD2","principal_investigator":[{"author_orcid":"0000-0002-4289-4810","middle_name":null,"organization":"School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow","street":"University Ave","town_or_city":"Glasgow","state_or_province":"Lanarkshire","post_or_zip":"G12 8QQ","telephone":null,"fax":null,"first_name":"Helen","last_name":"Walden","email":"helen.walden [at] glasgow.ac.uk","country":"United Kingdom","entry":"EMPIAR-11305"}],"status":"REL","deposition_date":"2022-11-14","release_date":"2022-12-05","obsolete_date":null,"update_date":"2022-12-05","corresponding_author":{"author":{"author_orcid":"0000-0002-0799-3450","middle_name":"Luke","organization":"School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow","street":"University Ave","town_or_city":"Glasgow","state_or_province":"Lanarkshire","post_or_zip":"G128QQ","first_name":"Martin","last_name":"Rennie","country":"United Kingdom"}},"authors":[{"author":{"name":"Rennie ML","author_orcid":"0000-0002-0799-3450"}},{"author":{"name":"Walden H","author_orcid":"0000-0002-4289-4810"}}],"cross_references":["EMD-11934"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rennie ML","author_orcid":"0000-0002-0799-3450"},{"name":"Arkinson C","author_orcid":"0000-0003-3665-2484"},{"name":"Chaugule VK","author_orcid":"0000-0001-7814-5865"},{"name":"Toth R","author_orcid":null},{"name":"Walden H","author_orcid":"0000-0002-4289-4810"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Structural basis of FANCD2 deubiquitination by USP1-UAF1","volume":"28","country":"","first_page":"356","last_page":"364","year":"2021","language":"English","doi":"10.1038/s41594-021-00576-8","pubmedid":"33795880","details":"Raw movie frames without alignment, without gain correction. Accumulating 65 electrons per squared Angstrom over 59 frames for an exposure time of 14.9 seconds. relion_convert_to_tiff was used to undo gain correction and convert MRCs to LZW compressed TIFFs.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":"4","preprint":false}],"dataset_size":"1.6 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7ay1"],"entry_doi":"10.6019/EMPIAR-11305"}}