{"EMPIAR-11299":{"imagesets":[{"segmentations":[],"name":"Unaligned multi-frame micrographs of reconstituted USP1, UAF1, FANCI, monoubiquitinated FANCD2, DNA, and ML323","directory":"data","category":"micrographs - multiframe","header_format":"TIFF","data_format":"TIFF","num_images_or_tilt_series":10998,"frames_per_image":40,"frame_range_min":null,"frame_range_max":null,"voxel_type":"UNSIGNED BYTE","pixel_width":1.06,"pixel_height":1.06,"micrographs_file_pattern":"data/data_*/*.tiff","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Each multi-frame raw micrograph is composed of 40 frames each in compressed TIFF format, along with a separate gain reference. Multi-frame micrographs were collected at 81,000x nominal magnification at 15.46 e-/pix/s in super-resolution mode with 2x binning on a Gatan K3. Data collected on KriosIV at eBIC on 2021-11-29. \nSample contained 9.3 μM USP1, 9.3 μM UAF1, 1.8 μM FANCI, 1.8 μM FANCD2 monoubiquitinated on K561, 2.2 μM dsDNA (61 base pairs), and 18.3 μM ML323.","image_width":"4092","image_height":"5760"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Reconstituted USP1, UAF1, FANCI, mono-ubiquitinated FANCD2 and the inhibitor ML323","principal_investigator":[{"author_orcid":"0000-0002-4289-4810","middle_name":null,"organization":"School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow","street":"University Ave","town_or_city":"Glasgow","state_or_province":"Lanarkshire","post_or_zip":"G12 8QQ","telephone":null,"fax":null,"first_name":"Helen","last_name":"Walden","email":"helen.walden [at] glasgow.ac.uk","country":"United Kingdom","entry":"EMPIAR-11299"}],"status":"REL","deposition_date":"2022-11-04","release_date":"2022-11-15","obsolete_date":null,"update_date":"2022-11-15","corresponding_author":{"author":{"author_orcid":"0000-0002-0799-3450","middle_name":"Luke","organization":"School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow","street":"University Ave","town_or_city":"Glasgow","state_or_province":"Lanarkshire","post_or_zip":"G128QQ","first_name":"Martin","last_name":"Rennie","country":"United Kingdom"}},"authors":[{"author":{"name":"Rennie ML","author_orcid":"0000-0002-0799-3450"}},{"author":{"name":"Walden H","author_orcid":"0000-0002-4289-4810"}}],"cross_references":["EMD-14719","EMD-14722","EMD-14720","EMD-15284","EMD-14721"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Rennie ML","author_orcid":"0000-0002-0799-3450"},{"name":"Arkinson C","author_orcid":"0000-0003-3665-2484"},{"name":"Chaugule VK","author_orcid":"0000-0001-7814-5865"},{"name":"Walden H","author_orcid":"0000-0002-4289-4810"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site","volume":"8","country":"","first_page":"eabq6353","last_page":"N/A","year":"2022","language":"English","doi":"10.1126/sciadv.abq6353","pubmedid":"36170365","details":"Raw movie frames without alignment, without gain correction. Accumulating 40 electrons per squared Angstrom over 40 frames at a rate of 15.46 electrons per pixel per second)","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Science advances","journal_abbreviation":"Sci Adv","issue":"39","preprint":false}],"dataset_size":"2.3 TB","experiment_type":"EMDB","scale":"molecule","related_pdb_entries":["7zh3","8a9j","7zh4"],"entry_doi":"10.6019/EMPIAR-11299"}}