{"EMPIAR-11277":{"imagesets":[{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 0% cholesterol","directory":"data/0CHOL_Arctica","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":615,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.14,"pixel_height":1.14,"micrographs_file_pattern":"data/0CHOL_Arctica/Micrographs/*.mrc","picked_particles_file_pattern":"data/0CHOL_Arctica/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 10% cholesterol","directory":"data/10CHOL_Arctica","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":653,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.14,"pixel_height":1.14,"micrographs_file_pattern":"data/10CHOL_Arctica/Micrographs/*.mrc","picked_particles_file_pattern":"data/10CHOL_Arctica/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 20% cholesterol","directory":"data/20CHOL_Artica","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":816,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.14,"pixel_height":1.14,"micrographs_file_pattern":"data/20CHOL_Artica/Micrographs/*.mrc","picked_particles_file_pattern":"data/20CHOL_Artica/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 30% cholesterol","directory":"data/30CHOL_Artica","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":492,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":1.14,"pixel_height":1.14,"micrographs_file_pattern":"data/30CHOL_Artica/Micrographs/*.mrc","picked_particles_file_pattern":"data/20CHOL_Artica/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 40% cholesterol","directory":"data/40CHOL_Arctica","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":481,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.715,"pixel_height":0.715,"micrographs_file_pattern":"data/40CHOL_Arctica/Micrographs/*.mrc","picked_particles_file_pattern":"data/40CHOL_Arctica/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.1 s per frame with an 11.5 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with lipid mixture containing POPC instead of SDPC","directory":"data/POPC_Arctica","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":502,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.715,"pixel_height":0.715,"micrographs_file_pattern":"data/POPC_Arctica/Micrographs/*.mrc","picked_particles_file_pattern":"data/POPC_Arctica/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.1 s per frame with an 11.5 s total exposure. Particle coordinates with refined alignments for the 34 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.","image_width":"11520","image_height":"8184"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer without any brominated lipids","directory":"data/NoBr_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":5684,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.835,"pixel_height":0.835,"micrographs_file_pattern":"data/NoBr_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/NoBr_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 5.9 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated cholesterol","directory":"data/CHOL-Br_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1855,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/CHOL-Br_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/CHOL-Br_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with iodinated cholesterol","directory":"data/CHOL-I_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3186,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/CHOL-I_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/CHOL-I_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated PIP2","directory":"data/PIP2-Br_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2118,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.835,"pixel_height":0.835,"micrographs_file_pattern":"data/PIP2-Br_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/PIP2-Br_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 5.9 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated POPS","directory":"data/POPS-Br_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":7806,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/POPS-Br_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/POPS-Br_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated SDPC","directory":"data/SDPC-Br_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2857,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/SDPC-Br_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/SDPC-Br_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4096"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 25% brominated SDPC","directory":"data/25SDPC-Br_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2931,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/25SDPC-Br_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/25SDPC-Br_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 50% brominated SDPC","directory":"data/50SDPC-Br_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":3891,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/50SDPC-Br_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/50SDPC-Br_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9A/F13A)/IST1 copolymer with brominated SDPC","directory":"data/SDPC-Br_F9A_F13A_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1715,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/SDPC-Br_F9A_F13A_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/SDPC-Br_F9A_F13A_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9E/F13E)/IST1 copolymer with brominated SDPC","directory":"data/SDPC-Br_F9E_F13E_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":1420,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/SDPC-Br_F9E_F13E_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/SDPC-Br_F9E_F13E_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"},{"segmentations":[],"name":"Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9L/F13L)/IST1 copolymer with brominated SDPC","directory":"data/SDPC-Br_F9L_F13L_Krios","category":"micrographs - single frame","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":2188,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"32 BIT FLOAT","pixel_width":0.822,"pixel_height":0.822,"micrographs_file_pattern":"data/SDPC-Br_F9L_F13L_Krios/Micrographs/*.mrc","picked_particles_file_pattern":"data/SDPC-Br_F9L_F13L_Krios/*.star","picked_particles_directory":"","details":"Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.","image_width":"5760","image_height":"4092"}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo Electron Microscopy of CHMP1B/IST1 Membrane-Bound Filaments with Brominated Lipid Probes","principal_investigator":[{"author_orcid":"0000-0003-2231-2577","middle_name":null,"organization":"University of California, San Francisco","street":null,"town_or_city":"San Francisco, CA","state_or_province":null,"post_or_zip":"94158","telephone":null,"fax":null,"first_name":"Adam","last_name":"Frost","email":"afrost [at] altoslab.com","country":"United States","entry":"EMPIAR-11277"}],"status":"REL","deposition_date":"2022-05-25","release_date":"2023-01-19","obsolete_date":null,"update_date":"2023-01-19","corresponding_author":{"author":{"author_orcid":"0000-0002-6149-6447","middle_name":"Russell","organization":"University of California, San Francisco","street":null,"town_or_city":"San Francisco, CA","state_or_province":null,"post_or_zip":"94110","first_name":"Frank","last_name":"Moss","country":"United States"}},"authors":[{"author":{"name":"Moss FR","author_orcid":"0000-0002-6149-6447"}},{"author":{"name":"Lincoff J","author_orcid":"0000-0003-3090-1240"}},{"author":{"name":"Tucker M","author_orcid":"0000-0002-1606-8806"}},{"author":{"name":"Mohammed A","author_orcid":"0000-0003-4430-7592"}},{"author":{"name":"Grabe M","author_orcid":"0000-0003-3509-5997"}},{"author":{"name":"Frost A","author_orcid":"0000-0003-2231-2577"}}],"cross_references":["EMD-28699","EMD-28714","EMD-28697","EMD-28717","EMD-28702","EMD-28712","EMD-28704","EMD-28696","EMD-28700","EMD-28719"],"biostudies_references":[],"idr_references":[],"empiar_references":[],"citation":[{"authors":[{"name":"Moss FR","author_orcid":"0000-0002-6149-6447"},{"name":"Lincoff J","author_orcid":"0000-0003-3090-1240"},{"name":"Tucker M","author_orcid":"0000-0002-1606-8806"},{"name":"Mohammed A","author_orcid":"0000-0003-4430-7592"},{"name":"Grabe M","author_orcid":"0000-0003-3509-5997"},{"name":"Frost A","author_orcid":"0000-0003-2231-2577"}],"editors":[{"name":"Frost A","author_orcid":"0000-0003-2231-2577"},{"name":"Moss FR","author_orcid":"0000-0002-6149-6447"}],"published":true,"j_or_nj_citation":true,"title":"Brominated lipid probes expose structural asymmetries in constricted membranes","volume":null,"country":"","first_page":null,"last_page":null,"year":"2023","language":"English","doi":"10.1038/s41594-022-00898-1","pubmedid":"36624348","details":"Motion-corrected micrographs of membrane-bound CHMP1B-IST1 filaments with and without brominated lipid probes.","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"Nature structural & molecular biology","journal_abbreviation":"Nat Struct Mol Biol","issue":null,"preprint":false}],"dataset_size":"3.7 TB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-11277"}}