{"EMPIAR-11264":{"imagesets":[{"segmentations":[],"name":"Single- and dual-axis tilt series used to perform segmented sub-tomogram averaging of microtubules assembled in the presence of GMPCPP and decorated with kinesin motor domains","directory":"data/raw_tilt_series","category":"tilt series","header_format":"MRC","data_format":"MRC","num_images_or_tilt_series":8,"frames_per_image":1,"frame_range_min":null,"frame_range_max":null,"voxel_type":"SIGNED 16 BIT INTEGER","pixel_width":null,"pixel_height":null,"micrographs_file_pattern":"","picked_particles_file_pattern":"","picked_particles_directory":"","details":"Single- and dual-axis tilt series. The number of images in the tilt series is variable. \nGMPCPP_S1_tomo1, GMPCPP_S1_tomo2, GMPCPP_S1_tomo3 and GMPCPP_S1_tomo4 are dual-axis tilt series, with a pixel spacing of 7.9 Å (image size 2048, 2048). \nGMPCPP_S2_tomo1 and GMPCPP_S2_tomo2 are single-axis tilt series, with a pixel size of 3.7 Å (image size 4096,4096). \nGMPCPP_tomoFig5 and GMPCPP_tomoFig6 are single-axis tilt series, with a pixel size of 2.1 Å (image size 4096,4096).","image_width":null,"image_height":null}],"workflow_file":null,"grant_references":[],"version_history":[],"title":"Cryo-electron tomography of microtubules assembled from purified porcine brain tubulin in the presence of GMPCPP","principal_investigator":[{"author_orcid":"0000-0001-8261-4396","middle_name":null,"organization":"Institute of Genetics and Development of Rennes","street":"263 avenue du Général Leclerc, Bâtiment 13","town_or_city":"Rennes","state_or_province":null,"post_or_zip":"35042","telephone":"+33 2 23 23 67 64","fax":null,"first_name":"Denis","last_name":"Chrétien","email":"denis.chretien [at] univ-rennes1.fr","country":"France","entry":"EMPIAR-11264"}],"status":"REL","deposition_date":"2022-08-29","release_date":"2022-11-14","obsolete_date":null,"update_date":"2023-01-12","corresponding_author":{"author":{"author_orcid":"0000-0001-8261-4396","middle_name":null,"organization":"Institute of Genetics and Development of Rennes","street":"263 avenue du Général Leclerc, Bâtiment 13","town_or_city":"Rennes","state_or_province":null,"post_or_zip":"35042","first_name":"Denis","last_name":"Chrétien","country":"France"}},"authors":[{"author":{"name":"Guyomar C","author_orcid":"0000-0001-9766-3932"}},{"author":{"name":"Bousquet C","author_orcid":"0000-0002-6905-0386"}},{"author":{"name":"Ku S","author_orcid":null}},{"author":{"name":"Heumann J","author_orcid":"0000-0001-6751-3028"}},{"author":{"name":"Guilloux G","author_orcid":"0000-0003-4674-3478"}},{"author":{"name":"Gaillard N","author_orcid":"0000-0002-1129-1291"}},{"author":{"name":"Heichette C","author_orcid":null}},{"author":{"name":"Duchesne L","author_orcid":"0000-0003-1985-6266"}},{"author":{"name":"Steinmetz MO","author_orcid":"0000-0001-6157-3687"}},{"author":{"name":"Gibeaux R","author_orcid":"0000-0001-5081-1985"}},{"author":{"name":"Chrétien D","author_orcid":"0000-0001-8261-4396"}}],"cross_references":["EMD-15740"],"biostudies_references":[],"idr_references":[],"empiar_references":[{"name":"EMPIAR-11253"}],"citation":[{"authors":[{"name":"Guyomar C","author_orcid":"0000-0001-9766-3932"},{"name":"Bousquet C","author_orcid":"0000-0002-6905-0386"},{"name":"Ku S","author_orcid":null},{"name":"Heumann J","author_orcid":null},{"name":"Guilloux G","author_orcid":"0000-0003-4674-3478"},{"name":"Gaillard N","author_orcid":"0000-0002-1129-1291"},{"name":"Heichette C","author_orcid":null},{"name":"Duchesne L","author_orcid":"0000-0003-1985-6266"},{"name":"Steinmetz MO","author_orcid":"0000-0001-6157-3687"},{"name":"Gibeaux R","author_orcid":"0000-0001-5081-1985"},{"name":"Chrétien D","author_orcid":"0000-0001-8261-4396"}],"editors":[],"published":true,"j_or_nj_citation":true,"title":"Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in <i>Xenopus</i> egg cytoplasmic extracts","volume":null,"country":"","first_page":null,"last_page":null,"year":"2021","language":"English","doi":"10.1101/2021.07.14.452321","pubmedid":null,"details":"Single and dual-axis tilt series of microtubules assembled from purified tubulin in the presence of GMPCPP, decorated with kinesin motor domains, and vitrified by plunge freezing into liquid ethane","book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"bioRxiv","journal_abbreviation":"","issue":null,"preprint":true},{"authors":[{"name":"Guyomar C","author_orcid":null},{"name":"Bousquet C","author_orcid":null},{"name":"Ku S","author_orcid":null},{"name":"Heumann JM","author_orcid":null},{"name":"Guilloux G","author_orcid":null},{"name":"Gaillard N","author_orcid":null},{"name":"Heichette C","author_orcid":null},{"name":"Duchesne L","author_orcid":null},{"name":"Steinmetz MO","author_orcid":null},{"name":"Gibeaux R","author_orcid":"0000-0001-5081-1985"},{"name":"Chrétien D","author_orcid":"0000-0001-8261-4396"}],"editors":[{"name":" ","author_orcid":null}],"published":true,"j_or_nj_citation":true,"title":"Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in <i>Xenopus</i> egg cytoplasmic extracts","volume":"11","country":"","first_page":null,"last_page":null,"year":"2022","language":"English","doi":"10.7554/elife.83021","pubmedid":"36503602","details":null,"book_chapter_title":null,"publisher":null,"publication_location":null,"journal":"eLife","journal_abbreviation":"Elife","issue":null,"preprint":false}],"dataset_size":"76.1 GB","experiment_type":"EMDB","scale":"molecule","entry_doi":"10.6019/EMPIAR-11264"}}